Features | Partner Sites | Information | LinkXpress
Sign In
RANDOX LABORATORIES
GLOBETECH PUBLISHING LLC
FOCUS DIAGNOSTICS, INC.

PCR Screening and Typing, Toolset Expanded for Ebolavirus

By Labmedica International staff writers
Posted on 19 Aug 2014
Image: Colorized transmission electron micrograph (TEM) revealing some of the ultrastructural morphology of an Ebolavirus virion (Image courtesy of Cynthia Goldsmith and the US Centers for Disease Control and Prevention (USA; CDC ref# PHIL 1832), via Wikimedia Commons).
Image: Colorized transmission electron micrograph (TEM) revealing some of the ultrastructural morphology of an Ebolavirus virion (Image courtesy of Cynthia Goldsmith and the US Centers for Disease Control and Prevention (USA; CDC ref# PHIL 1832), via Wikimedia Commons).
A new real-time polymerase chain reaction (RT-PCR)-based assay for identification of the five Ebolavirus species and Marburgvirus has been added to the Ebolavirus diagnostics toolset.

altona Diagnostics, GmbH (Hamburg, Germany), focused on the development, approval, manufacturing, and marketing of molecular diagnostic test systems for the detection and quantification of pathogens, has added a real-time RT-PCR-based assay, the RealStar Filovirus type RT-PCR kit, for identification of the five known Ebolavirus species and of Marburgvirus to their product portfolio. In addition to other activities, altona Diagnostics was also among the first to make reliable diagnostic kits commercially available during outbreaks for severe acute respiratory syndrome (SARS), avian Flu, swine Flu, and enterohemorrhagic Escherichia coli (EHEC).

Together with their already existing RealStar Filovirus Screen RT-PCR kit for first-line analysis, which is currently being used in the epidemic epicenter in West-Africa by the European Mobile Laboratory, altona Diagnostics now offers a comprehensive assay family for the detection and characterization of filovirus RNA from human sample material. While the screen kit detects all five Ebolavirus species without differentiation, as well as Marburgvirus, the typing kit is meant to be used as a second-line assay for confirmation of positive results and to distinguish between the five Ebolavirus species as well as Marburgvirus. Both kits are compatible with various real-time PCR platforms.

“For us, in the current situation during this epidemic outbreak, it´s more relevant that we were able to come up with a ready-to-use first-line test kit for filoviruses , which is now used in Guinea and Nigeria to identify infected patients and which is stocked by several European reference institutions under their preparedness plans,” said Dr. Markus Hess, chief scientific officer of altona Diagnostics, “The characterization of a positive sample by using the RealStar Filovirus type RT-PCR kit for Ebola and Marburgvirus is more of epidemiological and scientific importance.”

The ready-to-use real-time PCR kits for screening and typing of filoviruses are available as research use only reagents, currently. The In-Vitro Diagnostic (IVD) CE-marked version of the RealStar Filovirus Screen RT-PCR Kit will be available in September 2014.

Related Links:

altona Diagnostics
RealStar Filovirus RT-PCR kits



KARL HECHT GMBH & CO KG
77 ELEKTRONIKA
DIASYS DIAGNOSTIC SYSTEMS

Channels

Genetic Tests

view channel
Image: Globoid cell leukodystrophy: Mulinucleated macrophages (\"globoid cells\") and loss of myelinated fibers in a case of Krabbe\'s leukodystrophy (Photo courtesy of Wikimedia Commons).

Advanced Next-Generation Sequencing Assay Kit Now Available for the Diagnosis of Leukodystrophy

An advanced next-generation sequencing (NGS) assay kit is now available to augment the use of MRI scans to diagnose the class of brain disorders known as leukodystrophies. Leukodystrophies are genetic... Read more

Hematology

view channel
Image: The ADVIA 2120 Hematology System (Photo courtesy of Siemens).

Preoperative Blood Cell Ratios Predict Endometrial Cancer Survival

The variations in systemic inflammatory response biomarker levels have been associated with adverse clinical outcome in various malignancies and the host response to malignant tumors is characterized by... Read more

Immunology

view channel
Image: Long-lived plasma cells have a distinctive \"fried egg\" appearance, containing bubble-like vacuoles or lipid droplets, which are generally rare in bone marrow cell samples (Photo courtesy of Emory University).

Long-Lived Antibody-Producing Cells Identified in Bone Marrow

A distinct set of long-lived antibody-producing cells have been identified in the human bone marrow that function as an immune archive. These cells keep a catalog of how an adult's immune system responded... Read more

Lab Tech.

view channel
Image: Verigene System instrumentation consists of a Verigene Reader and one or multiple Verigene Processor SPs (Photo courtesy of Nanosphere).

Directed Molecular Diagnostic Tests Highlight Recent Microbiology Conference

The performance of a series of directed molecular diagnostic tests was confirmed by several studies presented at a recent microbiology conference. Nanosphere (Northbrook, IL, USA) manufactures tests... Read more

Industry News

view channel

Cancer Genetics Expands Access to Proprietary Diagnostics Through Agreement with Harvard Pilgrim

Under the terms of a new agreement between Cancer Genetics, Inc. (CGI; Morrisville, NC, USA) and Harvard Pilgrim Healthcare (Hartford, CT; USA), a health care network covering over one million people throughout Massachusetts, Connecticut, and Maine, CGI will offer its cancer diagnostic services to individuals covered under... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.