Features | Partner Sites | Information | LinkXpress
Sign In
SIEMENS DIAGNOSTICS

New Rapid Diagnostic Test Developed for Antibiotics Resistance

By Labmedica International staff writers
Posted on 08 Jul 2014
Image: Scanning electron micrograph of multidrug resistant Acinetobacter baumannii, a non-fermentative aerobic Gram-negative rod (Photo courtesy of Bioquell).
Image: Scanning electron micrograph of multidrug resistant Acinetobacter baumannii, a non-fermentative aerobic Gram-negative rod (Photo courtesy of Bioquell).
A rapid diagnostic test for multiresistance to broad-spectrum antibiotics has been developed for multidrug-resistant strains of Acinetobacter baumannii, an important hospital pathogen.

Bacterial resistance to antibiotics has increased considerably over recent years and the situation is particularly dramatic in regard to Gram-negative bacilli such as Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and A. baumannii, found in septicemic and abdominal infections and infections of the urinary tract and the lungs.

Microbiologists at the University of Fribourg (Switzerland) and their colleagues at Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, France) collected a total of 220 strains to evaluate the performance of the CarbAcineto NP test. The strains included a collection of 151 carbapenemase-producing and 69 non-carbapenemase-producing Acinetobacter spp.

The test is based on the acidification properties generated by the enzymatic hydrolysis of a carbapenem, imipenem, when it is cleaved by a carbapenemase. The medium acidifies and the acidity indicator, which indicates pH, then turns from red to yellow. The detection of this carbapenemase activity can be realized by testing already isolated bacteria or any site infections. The result is obtained in less than two hours, while other techniques currently available require a minimum of 24 hours, most frequently 72 hours.

The specificity and of the CarbAcineto NP test were found to be 100% and the sensitivity was 94.7%. The CarbAcineto NP test efficiently detected OXA-type carbapenemase producers, leading to a significant improvement of the sensitivity to 94.7% for the CarbAcineto NP test versus 11.9% for the original Carba NP test. The use of the CarbAcineto NP test will be particularly important for intensive care unit (ICU) patients, for whom multidrug-resistant isolates belonging to the A. baumannii-A. calcoaceticus complex are a common source of severe infections. It specifically makes sense to test for carbapenemase production considering that the acquisition of a carbapenemase in A. baumannii is a marker of multidrug resistance.

The authors concluded that the CarbAcineto NP test is simple, cost-effective and, by detecting multidrug-resistant strains, it can prevent them spreading via outbreaks of hospital infections caused by multidrug-resistant bacteria, particularly among the most seriously ill patients and those undergoing resuscitation. This new test also provides a guide in the choice between the very few remaining treatment options for infected patients. Inserm Transfert (Paris, France) have filed an international patent for the CarbAcineto NP test. The study was published in the May 2014 issue of the Journal of Clinical Microbiology.

Related Links:

University of Fribourg
Faculté de Médecine Paris-Sud 
Inserm Transfert



comments powered by Disqus

Channels

Immunology

view channel
Image: The fluorescence-activated cell sorting FACSCanto II flow cytometer (Photo courtesy of BD Biosystems).

Flow Cytometry Detects Lymphoproliferative Disorders in Fluid Specimens

Immunophenotypic analysis of hematopoietic cell populations by flow cytometry has emerged as a useful ancillary study in the diagnostic evaluation of serous effusions and cerebrospinal fluids (CSFs).... Read more

Pathology

view channel

Pathologists Publish Recommendations for Prostate Cancer Active Surveillance

Pathologists have developed a set of recommendations to help effectively diagnose low-risk prostate cancer (PrCa) patients eligible for active surveillance and less harmful treatment options. Active surveillance offers low-risk PrCa patients a means to avoid the potentially harmful side-effects from treatment.... Read more

Lab Tech.

view channel
Image: A technical breakthrough for DNA imaging has been achieved that should quicken diagnosis of diseases for which analysis of DNA from single-cells is critical, such as early stage cancers and various pre-natal conditions (Photo courtesy of McGill University and Génome Québec Innovation Center).

Breakthrough DNA Analysis Technology to Hasten Problem Diagnosis

Researchers have achieved a technical breakthrough that should result in speedier diagnosis of diseases for which analysis of DNA from single-cells is critical, such as early stage cancers and various... Read more

Industry News

view channel

Most IVD Sector Companies Look Outside the EU and USA for Growth

Kalorama Information (New York City, NY, USA) has now published the 9th edition of its biennial market research report “Worldwide Market for In Vitro Diagnostics,” revealing estimates from its survey of the in vitro diagnostics (IVD) industry – presenting the trends, technologies, customer needs, and major suppliers with... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.