Features | Partner Sites | Information | LinkXpress
Sign In
FOCUS DIAGNOSTICS, INC.
RANDOX LABORATORIES
GLOBETECH PUBLISHING LLC

Effective Way to Diagnose Latent Tuberculosis Identified

By Labmedica International staff writers
Posted on 05 Jun 2014
Image: The QuantiFERON-TB Gold In-Tube whole blood enzyme-linked immunosorbent assay (ELISA) for the diagnosis of tuberculosis (Photo courtesy of Cellestis).
Image: The QuantiFERON-TB Gold In-Tube whole blood enzyme-linked immunosorbent assay (ELISA) for the diagnosis of tuberculosis (Photo courtesy of Cellestis).
The effective control and elimination of tuberculosis (TB) in low-incidence countries requires the prompt identification of individuals with active and latent TB infection (LTBI).

The diagnosis of latent TB is important because the bacterium can persist in the body only to emerge and reactivate months or even years later. Therefore treating individuals with latent TB can prevent disease developing and so prevent them infecting others.

Scientists at University of Birmingham (Edgbaston, UK) working with colleagues from other British institutes performed a prospective, longitudinal cohort study among 166 Nepalese military recruits who had left Nepal and recently entered the UK. The study was carried out from February to September 2012, and assessed longitudinal interferon (IFN)-gamma release assays (IGRA) and tuberculin skin test (TST) responses. They also studied the influence of antecedent TST and LTBI treatment on IGRA performance.

Two commercially available assays were used. The QuantiFERON-TB Gold In-Tube (QFT-GIT; Cellestis; Carnegie, Australia) a whole blood enzyme-linked immunosorbent assay (ELISA), and the T-SPOT.TB assay (Oxford Immunotec; Abingdon, UK) enzyme-linked immunospot (ELISPOT) assay using peripheral blood mononuclear cells (PBMCs). On the day following blood sampling for the IGRAs, a TST was performed by the Mantoux method.

The IGRA testing revealed that 21 individuals were T-SPOT.TB positive (12.9%, with two who were borderline positive, and eight were QFT-GIT positive (4.8%). All QFT-GIT positives were also T-SPOT.TB positive. Thus, 61.9% T-SPOT.TB positive participants were QFT-GIT negative, meaning the tests showed moderate agreement.

Adam F. Cunningham, PhD, the senior author of the study and a professor of Functional Immunity, said, “One of the problems with latent TB is that it is very tricky to diagnose. We have found this test is the best way for diagnosing latent TB in this group. What we found was that one IGRA was more effective at identifying latent TB infected individuals than the other tests. This commercially-produced test, called T-Spot, is a blood test that measures T cells specific to certain Mycobacterium tuberculosis antigens. It is cost-effective because individuals only require one visit to be tested.” The study was published on May 9, 2014, in the journal Public Library of Science ONE.

Related Links:

University of Birmingham
Cellestis 
Oxford Immunotec 



KARL HECHT GMBH & CO KG
CELLAVISION AB
MEDLAB Asia
PERIPHERAL VISIONS INC

Channels

Clinical Chemistry

view channel
Image: UniCel DxC 800 Synchron Clinical Systems (Photo courtesy of Beckman Coulter).

Routine Blood Glucose Value Correlates with Diabetes Risk

Random glucose values obtained during routine blood tests are often overlooked, but could provide valuable insight into whether someone is at risk for having type 2 diabetes. Random Blood Glucose (RBG)... Read more

Genetic Tests

view channel
Image: Histopathology of tuberculoid leprosy in a skin section (Photo courtesy Dr. D.S. Ridley, Wellcome Images).

Genes Discovered Influence Risk of Developing Leprosy

Leprosy, a chronic dermatological and neurological disease, is caused by infection with Mycobacterium leprae, and its manifestation, progression and prognosis are strongly associated with the proficiency... Read more

Hematology

view channel
Image: Plastic bag containing 0.5 to 0.7 liters of packed red blood cells in citrate, phosphate, dextrose, and adenine (CPDA) solution (Photo courtesy of Fresenius HemoCare).

Transfusion Protocols Compared After Cardiac Surgery

Unnecessary blood transfusions may increase healthcare costs both directly, because blood is an increasingly scarce and expensive resource, and indirectly due to the complications associated with transfusion.... Read more

Industry News

view channel

Sequencing-Based Testing Sector Already Highly Competitive

As next-generation sequencing (NGS) reaches the clinical laboratory, a new analysis by Kalorama Information (New York, NY, USA) finds that small reference laboratories as well as over 50 companies now offer sequencing-based testing through their own CLIA-certified laboratories, some of which offer a wide range of tests.... Read more
 

Events

28 Mar 2015 - 01 Apr 2015
06 Apr 2015 - 08 Apr 2015
07 Apr 2015 - 09 Apr 2015
Copyright © 2000-2015 Globetech Media. All rights reserved.