Features | Partner Sites | Information | LinkXpress
Sign In
PURITAN MEDICAL
Bio-Rad Diabetes Division
FOCUS DIAGNOSTICS, INC.

New Test Rapidly Identifies Platelet Function Defects

By Labmedica International staff writers
Posted on 07 Aug 2014
Image: Photomicrograph of a blood smear showing a giant platelet (arrowed) from a patient with hemorrhagiparous thrombocytic dystrophy (Photo courtesy of the College of American Pathologists).
Image: Photomicrograph of a blood smear showing a giant platelet (arrowed) from a patient with hemorrhagiparous thrombocytic dystrophy (Photo courtesy of the College of American Pathologists).
A multisurface and multiparameter flow assay to characterize thrombus formation in whole blood from healthy subjects and patients with platelet function deficiencies has been created.

Parallel-plate flow chambers have been used for the last two decades to measure platelet adhesion and activation under arterial or venous flow conditions, in particular using surfaces such as extracellular matrix or collagen, but there is a need for further standardization of devices, protocols and measurement parameters.

A team of scientists led by those at Maastricht University (The Netherlands) obtained blood samples from healthy donors and patients who all had a well-defined platelet deficiency and mild bleeding tendency. Microspot-coated coverslips were mounted onto a transparent parallel-plate flow chamber (50 μm depth, 3 mm width and 20 mm length), and pre-rinsed. Anticoagulated whole-blood samples were perfused through the flow chamber for a time period sufficient for full-thrombus formation on collagen I spots and adherence labeled with a fluorescence marker.

A systematic comparison is made of 52 adhesive surfaces with components activating the main platelet-adhesive receptors, and of eight output parameters reflecting distinct stages of thrombus formation. Three types of thrombus formation can be identified with a predicted hierarchy of the following receptors: glycoprotein (GP) VI, C-type lectin-like receptor-2 (CLEC-2) > GPIb > α6β1 αIIbβ3 > α2β1 > CD36, α5β1, αvβ3. Application with patient blood reveals distinct abnormalities in thrombus formation in patients with severe combined immune deficiency, Glanzmann’s thrombasthenia, Hermansky–Pudlak syndrome, May–Hegglin anomaly or grey platelet syndrome. This test may be useful for the diagnosis of patients with suspected bleeding disorders or a pro-thrombotic tendency.

Johan W.M. Heemskerk, PhD, a professor and a senior author of the study said, “To develop this test we collected information on thrombosis formation in a flow chamber with flowing blood. This has never been done before on such a large scale. We can easily determine what’s wrong with the patient by counting the number of platelets in a few drops of blood. If there’s an active bleed, the platelets trigger the coagulation process to stem the bleeding. The opposite is true with thrombosis: in that case, the platelets are overactive and could cause a stroke or a heart attack.” The study was published on July 16, 2014, in the journal Nature Communications.

Related Links:

Maastricht University



KARL HECHT GMBH & CO KG
DIASYS DIAGNOSTIC SYSTEMS
ADVANCED INSTRUMENTS
WATERS CORPORATION

Channels

Genetic Tests

view channel

Overexpression of X Chromosome Genes Is Biomarker for Female Mental Disorders

A recent paper suggested that elevated expression of the genes XIST (X-inactive specific transcript) and KDM5C (lysine-specific demethylase 5C) could be used as a biological marker for diagnosis of major affective disorders in a significantly large subset of female patients from the general population. Xist is an RNA... Read more

Immunology

view channel

Powerful Innovations in Diagnostics Unveiled at EuroMedLab 2015

New systems presented by Roche include a new standard for immunology testing, an integrated platform for hematology diagnostics, and a high-throughput analyzer to perform HbA1c tests for blood glucose management. Also, evidence was presented for a high-sensitivity cardiac troponin test to provide earlier and more accurate... Read more

Industry News

view channel

Cloud-Based Systems Becoming Key Pillar in eHealth Strategy

Cloud-based laboratory information management systems (LIMS) are becoming a key pillar in Europe’s e-Health strategy as data-management solutions for modern testing will generate more demand for these systems. Investments in cloud-based LIMS are part of Europe’s e-Health strategy. New analysis from Frost & Sullivan... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.