Features | Partner Sites | Information | LinkXpress
Sign In
RANDOX LABORATORIES
FOCUS DIAGNOSTICS, INC.
GLOBETECH PUBLISHING LLC

Automated Slidemaker-Stainer Evaluated for Performance

By Labmedica International staff writers
Posted on 01 May 2014
Image: UniCel DxH Slidemaker Stainer Coulter Cellular Analysis System (Photo courtesy of Beckman Coulter).
Image: UniCel DxH Slidemaker Stainer Coulter Cellular Analysis System (Photo courtesy of Beckman Coulter).
Although there have been advancements in instrumentation within hematology laboratories, there is still a need for review of a peripheral blood films (PBF).

Morphological assessment is extremely subjective and dependent on the acceptable standard of the film being assessed, and it is important to determine whether or not automated slidemaker-stainers are able to consistently and reproducibly prepare and stain blood films of exemplary quality, without carryover between specimens.

Hematologists at the London Health Sciences Center (London, ON, Canada) selected a total of 131 specimens, 46 morphologically normal and 85 hematologically abnormal, from their Health Center which specializes in cancer care, pediatrics, and obstetrics. A broad specimen pool of potential disease states was recruited from the day-to-day workload over an eight-day period. Four blood films were prepared manually and stained with the laboratory's routine method of Wright-Giemsa.

The scientists evaluated the UniCel DxH slidemaker stainer (DxH SMS, Beckman Coulter; Brea, CA, USA) which is a fully automated, integrated slidemaker, and stainer intended for the hematology laboratory. The DxH SMS is available either as a stand-alone instrument or part of the DxH workcell configuration. A unique patented device, the hemasphere, is incorporated into the system to measure “residual clinging” of the blood sample to an internal surface as an analogue for the way in which blood would behave as it is spread on a glass slide. Two samples were selected to perform this study evaluating the degree of cellular carryover that has the potential to occur in circumstances when a sample with extreme leukocytosis is followed by one with extreme leukopenia.

The investigators found that carryover was not an issue and repeatability was within expected limits. There was excellent agreement of the five-part differential between the automated blood films made by the DxH-SMS compared with the manually prepared reference blood film. There was no difference in identification and enumeration of blasts, variant lymphocytes, or nucleated red blood cells and red cell morphology showed excellent agreement.

The authors concluded that blood films prepared by the DxH-SMS are of excellent quality, the required cleaning requirements are simple, the software is intuitive, and the burden of quality related to the reagents is carried by the manufacturer. All of these reasons led them to believe they have made the right choice in incorporating the slidemaker/stainer into the configuration of their clinical instrumentation. The study was published in the April 2014 issue of International Journal of Laboratory Hematology.

Related Links:
London Health Sciences Center
Beckman Coulter



CELLAVISION AB
MEDLAB Asia
KARL HECHT GMBH & CO KG
PERIPHERAL VISIONS INC

Channels

Clinical Chemistry

view channel
Image: UniCel DxC 800 Synchron Clinical Systems (Photo courtesy of Beckman Coulter).

Routine Blood Glucose Value Correlates with Diabetes Risk

Random glucose values obtained during routine blood tests are often overlooked, but could provide valuable insight into whether someone is at risk for having type 2 diabetes. Random Blood Glucose (RBG)... Read more

Genetic Tests

view channel
Image: Histopathology of tuberculoid leprosy in a skin section (Photo courtesy Dr. D.S. Ridley, Wellcome Images).

Genes Discovered Influence Risk of Developing Leprosy

Leprosy, a chronic dermatological and neurological disease, is caused by infection with Mycobacterium leprae, and its manifestation, progression and prognosis are strongly associated with the proficiency... Read more

Industry News

view channel

Sequencing-Based Testing Sector Already Highly Competitive

As next-generation sequencing (NGS) reaches the clinical laboratory, a new analysis by Kalorama Information (New York, NY, USA) finds that small reference laboratories as well as over 50 companies now offer sequencing-based testing through their own CLIA-certified laboratories, some of which offer a wide range of tests.... Read more
 

Events

28 Mar 2015 - 01 Apr 2015
06 Apr 2015 - 08 Apr 2015
07 Apr 2015 - 09 Apr 2015
Copyright © 2000-2015 Globetech Media. All rights reserved.