We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Abbott Diagnostics

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
06 Jun 2020 - 09 Jun 2020
Virtual Venue
08 Jun 2020 - 11 Jun 2020
Virtual Venue

LAMP Malaria Test Detects Less-Diagnosed Species

By LabMedica International staff writers
Posted on 16 May 2019
Print article
Image: The HumaLoop M: The easy-to-use Loopamp technology for primary and district laboratories (Photo courtesy of Human Diagnostics).
Image: The HumaLoop M: The easy-to-use Loopamp technology for primary and district laboratories (Photo courtesy of Human Diagnostics).
A new loop-mediated isothermal amplification (LAMP) molecular assay has been developed that can detect Plasmodium vivax, a species of the parasite that causes malaria.

Almost all molecular tests currently available detect P. falciparum, in part because it causes the highest mortality of the different species. However, P. vivax is actually more widespread, with more than 2.5 billion people at risk of infection.

The new LAMP assay was evaluated recently in Colombia using a protocol of consecutive testing, a pan-plasmodium LAMP test followed by the P. vivax test, with polymerase chain reaction (PCR)-based testing as the comparator. In all, 365 fingerpick blood samples from patients with fever were evaluated, and the P. vivax test showed a sensitivity of approximately 94% and a specificity of 92%. Importantly, the test also has a low limit of detection of around one to two parasites per microliter of patient sample.

The LAMP assay was developed by Eiken Chemical (Tokyo, Japan) with the collaboration of The Foundation for Innovative New Diagnostics (FIND, Geneva, Switzerland) who coordinated laboratory testing in collaboration with the Hospital for Tropical Diseases (London, UK). FIND has negotiated pricing for the Loopamp tests and HumaTurb system, with a kit of 96 P. vivax tests costing EUR 499.20 (USD 560) and the HumaTurb costing EUR 15,900. There is also a solution for lower-throughput settings called HumaLoop that is a lower cost.

Xavier C. Ding, PhD, the team leader for malaria diagnostics at FIND, said, “It's only recently that there is a recognition that if we are ever to eliminate malaria, we also need some vivax-specific tests, because that species has its own biological specificities and is much more challenging to eliminate that falciparum.” This is because, unlike P. falciparum, P. vivax can lie dormant in the liver of an infected person in a stage called a hypnozoite, and a person with hypnozoites is then prone to relapsing malarial disease. Dr. Ding added, “There is big hope that this will make it easier and more accessible to get treatment for liver-stage parasites, but that also means you also need good diagnostic tools to identify those people who have vivax.”

Related Links:
Eiken Chemical
The Foundation for Innovative New Diagnostics
Hospital for Tropical Diseases

Print article


Lab Tech.

view channel
Image: Ultra-fast Cycling for Multiplexed Cellular Fluorescence Imaging (Photo courtesy of Massachusetts General Hospital Research Institute).

Ultra-Fast Cycling for Multiplexed Cellular Fluorescence Imaging

In the treatment of tumors, microenvironment plays an important role. It often contains immune cells that are so changed that they promote tumor growth. Until now, analyses of these dynamic changes with... Read more
Copyright © 2000-2020 Globetech Media. All rights reserved.