We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Liquid Biopsy Identifies Breast Cancer Patients Destined to Recur

By LabMedica International staff writers
Posted on 15 Jun 2022
Print article
Image: Histopathology of hormone receptor–positive (HR+) breast cancer (Photo courtesy of Emily S. Reisenbichler, MD)
Image: Histopathology of hormone receptor–positive (HR+) breast cancer (Photo courtesy of Emily S. Reisenbichler, MD)

Breast cancer is the most commonly diagnosed cancer worldwide. Most breast cancers, nearly 80%, are hormone receptor–positive (HR+). Unlike other subtypes of breast cancers in which risk of recurrence decreases 5 years after diagnosis, the recurrence risk for HR+ breast cancer remains steady from 5 years to at least 20 years with some patients experiencing recurrence three decades after diagnosis.

Most recurrences of hormone receptor–positive breast cancers (HR+ BCs) occur in the late adjuvant setting. Clinicopathologic tools in routine practice are insufficient to determine who remains at high risk of late recurrence. Detection of minimal residual disease (MRD) via plasma circulating tumor DNA (ctDNA) is promising yet not well understood in this population.

Medical Oncologists at the Dana-Farber Cancer Institute (Boston, MA, USA) and their colleagues enrolled 103 patients with high-risk stage II-III HR+ BC diagnosed more than 5 years prior without clinical evidence of recurrence. Serial blood samples were collected every 6-12 months at time of scheduled patient visits. DNA was extracted from buffy coat, and circulating cell-free DNA was extracted from plasma.

Archival tumor tissue was obtained from initial breast cancer surgery. Formalin-fixed paraffin-embedded tissue block and Hematoxylin & Eosin slides were made and DNA was extracted and whole-exome sequencing (WES) performed. Detection of somatic mutations was used to design, for each patient, a personalized ctDNA Rapid Automatic Detection and Alignment of Repeats in protein sequences (RaDaR). WES data were reanalyzed to identify mutations in oncogenes for the comutation plot.

The investigators reported that In total, 85 of 103 patients had sufficient tumor tissue; of them, 83 of 85 (97.6%) patients had successful whole-exome sequencing. Personalized ctDNA assays were designed targeting a median of 36 variants to test 219 plasma samples. The median time from diagnosis to first sample was 8.4 years. The median follow-up was 10.4 years from diagnosis and 2.0 years from first sample. The median number of plasma samples per patient was two. Eight patients (10%) had positive MRD testing at any time point. Six patients (7.2%) developed distant metastatic recurrence, all of whom were MRD-positive before overt clinical recurrence, with median ctDNA lead time of 12.4 months. MRD was not identified in one patient (1.2%) with local recurrence. Two of eight MRD-positive patients had not had clinical recurrence at last follow-up.

Marla Lipsyc-Sharf, MD, an Oncologist and lead author of the study, said, “If only one or a few of the mutations were tracked for each patient, not all of the eight MRD-positive patients in this cohort would have been identified.”

The authors concluded that detection of MRD was strongly associated with distant metastatic recurrences more than 5 years from breast cancer diagnosis, with favorable test characteristics including sensitivity, specificity, and a median lead time of approximately 1 year to clinical recurrence. These data suggest that there may be a period in which MRD is detectable via ctDNA before overt, late breast cancer recurrences. The study was published on June 4, 2022 in the Journal of Clinical Oncology.

Related Links:
Dana-Farber Cancer Institute 

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
HLX
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
New
Gold Member
Plasma Control
Plasma Control Level 1

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: MOF materials efficiently enrich cfDNA and cfRNA in blood through simple operational process (Photo courtesy of Science China Press)

Blood Circulating Nucleic Acid Enrichment Technique Enables Non-Invasive Liver Cancer Diagnosis

The ability to diagnose diseases early can significantly enhance the effectiveness of clinical treatments and improve survival rates. One promising approach for non-invasive early diagnosis is the use... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.