We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

D-Serine Clearance Evaluates Precise Measurement of Kidney Function

By LabMedica International staff writers
Posted on 21 Dec 2021
Print article
Image: D-Serine is useful for the measurement of glomerular filtration rate (GFR). The clearance of D-serine has an advantage of lower bias against GFR compared to that with the creatinine clearance. Combinational clearance measurement of D-serine and creatinine can serve as a measure of GFR with precision and low bias (Photo courtesy of Tomonori Kimura, MD, PhD)
Image: D-Serine is useful for the measurement of glomerular filtration rate (GFR). The clearance of D-serine has an advantage of lower bias against GFR compared to that with the creatinine clearance. Combinational clearance measurement of D-serine and creatinine can serve as a measure of GFR with precision and low bias (Photo courtesy of Tomonori Kimura, MD, PhD)
Evaluation of kidney function is essential in daily clinical practice. The measured glomerular filtration rate (mGFR) using exogenous inulin has been considered as the gold standard of kidney function, though this procedure is labor intensive, time consuming, and expensive.

D-Serine is a candidate biomarker of kidney function. D-Serine circulates the bloodstream in the body and is delivered to the kidney. D-Serine is then filtrated through the glomerulus and excreted in the urine. When kidney function is impaired, the urinary excretion of D-serine decreases, and its blood level increases.

Medical Scientists at the Osaka University (Osaka, Japan) and their colleagues carried out a cross-sectional observational study of 200 living kidney transplant donors and recipients enrolled between July 2019 and December 2020, for whom GFR was measured by clearance of inulin (C-in).

The clearance of D-serine (C-DSer) was calculated based on blood and urine levels of D-serine, as measured by two-dimensional high-performance liquid chromatography. Analytical performance was assessed by calculating biases. Utilizing data from 129 participants, the team developed equations for C-in based on C-DSer and C-cre using a linear regression model, and the performance was validated in 68 participants. Creatinine in serum and urine was measured enzymatically (Determiner L CRE, Hitachi Chemical, Tokyo, Japan), and serum cystatin C (sCys) was measured using an immunological turbid metric assay (Nescoat GC Cystatin C, Alfresa Pharma, Osaka, Japan).

The investigators reported that in the overall cohort, the mean values of serum creatinine, cystatin C, and plasma D-serine were 0.86 mg/dL, 1.1 mg/L, and 2.3 μM, respectively. The mean clearances of inulin, creatinine, and D-serine were 66.7, 98.2, and 55.7mL per minutes per 1.73m2 of body surface area, respectively. The levels and clearances of D-serine in plasma and serum were almost identical and so they used plasma level of D-serine in the analysis. In the total cohort population, C-DSer was significantly and strongly correlated with C-in (R = 0.91), like C-cre (R = 0.93). Remarkably, the clearance of D-serine agreed well with GFR. The low bias as a measure of GFR was an advantage for the D-serine clearance. The degree of bias against GFR was smaller than that of the creatinine clearance. Additionally, the combinational analysis of clearances of D-serine and creatinine could measure GFR with high performance.

Tomonori Kimura, MD, PhD, the senior author of the study, said, “D-Serine turned out to be of great clinical importance. D-Serine may solve the problem of kidney disease with more than 800 million patients in the world. Measuring D-serine is applicable in a wide range of clinical fields and for drug development.”

The authors concluded that the combination of C-DSer and C-cre can measure the GFR with precision and minor biases. The current method using endogenous molecules may reduce clinical burdens for measuring GFR. The new method is precise across the tested range of GFR and has the potential to facilitate key clinical decisions in various clinical situations. The study was published on December 4, 2021 in the journal EClinicalMedicine.

Related Links:
Osaka University
Hitachi Chemical
Alfresa Pharma


Automated ELISA-IFA-BLOT Processor AP 22
Gold Supplier
SARS-CoV-2 (ORF1a, N, RdRp) Real-Time RT-PCR Test
DiaPlexQ SARS-CoV-2 (ORF1a, N, RdRp)
New
Automated Hematology Analyzer
Celltac Alpha MEK-1301/1302
New
Platinum Supplier
Hormonal Test
Endocrine Array

Print article

Channels

Molecular Diagnostics

view channel
Image: A new method reliably detects protein changes in blood that are typical of Parkinson`s disease (Photo courtesy of Pexels)

First-Ever Blood Test Detects Parkinson’s Disease

Until now, the diagnosis of Parkinson's disease has been based primarily on typical movement disorders such as muscle stiffness, slower movements and shaking. However, the disease starts up to 20 years... Read more

Industry

view channel
Image: Fujirebio has acquired ADx NeuroSciences for 40 million Euros (Photo courtesy of Pexels)

Fujirebio Acquires ADx NeuroSciences to Speed Development of Neurodegenerative Diseases Diagnostic Tests

Fujirebio Holdings, Inc. (Tokyo, Japan) has announced the acquisition of ADx NeuroSciences (Gent, Belgium) for EUR 40 million in a deal that is expected to close in July 2022, pending the satisfaction... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.