We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Olympus

Manufactures optical and digital equipment for the healthcare and consumer electronics sectors, including endoscopy a... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

CD8 T-Cell–Predominant Lymphocytic Esophagitis Associated with GERD

By LabMedica International staff writers
Posted on 05 Oct 2021
Print article
Image: Histopathology photomicrograph of lymphocytic esophagitis: Esophageal mucosa showing peripapillary intraepithelial lymphocytosis with basal zone hyperplasia and intercellular edema. No significant population of eosinophils or neutrophils is identified (Photo courtesy of Yusuf Kasirye, MD, et al)
Image: Histopathology photomicrograph of lymphocytic esophagitis: Esophageal mucosa showing peripapillary intraepithelial lymphocytosis with basal zone hyperplasia and intercellular edema. No significant population of eosinophils or neutrophils is identified (Photo courtesy of Yusuf Kasirye, MD, et al)
In patients with reflux esophagitis (RE), increased lymphocytes are often part of a mixed inflammatory infiltrate that also includes eosinophils and/or neutrophils. Less frequently, lymphocytes are the only type of inflammatory cells associated with gastroesophageal reflux disease (GERD).

One such pattern is lymphocytic esophagitis (LyE), which is characterized by an elevated number of peripapillary lymphocytes and absent or rare intraepithelial granulocytes. This pattern has been reported in approximately 5% of patients with endoscopic esophagitis and 7% of patients with Barrett esophagus.

Clinical Scientists at the Dartmouth-Hitchcock Medical Center (Lebanon, NH, USA) conducted an observational retrospective study and identified 161 patients seen at their institution from 1998 to 2014 who were diagnosed with GERD, had normal esophageal motility, and available esophageal biopsies. For all patients meeting inclusion criteria, the team obtained demographic data as well as information pertaining to clinical diagnosis, past medical history, and endoscopic, imaging, manometry, and, where available, pH-metry findings from the files and electronic medical records.

Biopsy specimens were fixed in 10% formalin, paraffin-embedded, and stained with hematoxylin-eosin. A single peripapillary lymphoid infiltrate was sufficient for the diagnosis of LyE. The cutoffs for a normal number of intraepithelial lymphocytes evaluated in hematoxylin-eosin–stained slides at different levels, such as gastroesophageal junction, distal esophagus, and midesophagus were 62, 46, and 41 lymphocytes per high-power field, respectively. Cells were counted in one mostly affected high-power field using an Olympus BX 41 microscope (Olympus, Center Valley, PA, USA). Routine CD4 and CD8 immunohistochemistry was performed using Bond Polymer Refine Detection staining reagents and Bond III autostainer (Leica Microsystems, Buffalo Grove, IL, USA).

The scientists found increased intraepithelial lymphocytes in 13.7% of patients with GERD. Two major patterns and one minor pattern of lymphocytic inflammation were observed as follows: (1) LyE (in 6.8% [11 of 161] of patients and typically focal), (2) dispersed lymphocytes in an area of reflux esophagitis (in 5.6% [9 of 161] and typically diffuse), and (3) peripapillary lymphocytes in an area of reflux esophagitis (in 1.2% [2 of 161]). CD8 T cells significantly outnumbered CD4 T cells in 91% of patients with lymphocytic esophagitis and 100% of patients with dispersed lymphocytes (9 of 9) or peripapillary lymphocytes (2 of 2) in the area of reflux esophagitis.

The authors concluded that their findings suggest that LyE is one of the major patterns of lymphocytic inflammation in GERD. CD8 T-cell–predominant immunophenotype may be useful as a marker of GERD in the differential diagnosis of LyE. The study was published in the September 2021 issue of the journal Archives of Pathology and Laboratory Medicine.

Related Links:
Dartmouth-Hitchcock Medical Center
Olympus
Leica Microsystems


Gold Supplier
NEW PRODUCT : SILICONE WASHING MACHINE TRAY COVER WITH VICOLAB SILICONE NET VICOLAB®
REGISTRED 682.9
New
Pancreatic Elastase ELISA Kit
Pancreatic Elastase ELISA
New
Respiratory Multiplex RT-PCR Kit
Respiratory Multiplex RT-PCR Kit
New
6-Channel Viscoelastometry Analyzer
ClotPro

Print article

Channels

Molecular Diagnostics

view channel
Image: Absence of nuclear immunohistochemical staining of MSH2 protein (A) and presence of MLH1 protein (B) in urothelial cell carcinoma of the urinary bladder of a patient carrying a germline MSH2 mutation. Observe the nuclear staining in stromal cells as an internal control (Photo courtesy of Radboud University Nijmegen Medical Centre)

Simple Urine Test Detects Urothelial Cancers in Lynch Syndrome Patients

Lynch Syndrome (LS) is an inherited genetic disorder that carries a high risk of cancer. LS is caused by mutations affecting MLH1, MSH2, MSH6 or PMS2 genes. More than one in 300 people have LS but most... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test for screening, diagnosis and management of sickle cell disease and related hemoglobinopathies at the point of care (Photo courtesy of Hemex Health)

Point-of-Care Device Accurately Rapidly Diagnoses Sickle Cell Disease

Hemoglobinopathies are the most common autosomal hereditary disorders. Approximately 7% of the global population carries hemoglobin gene mutation including structural hemoglobin variants like sickle hemoglobin... Read more

Immunology

view channel
Image: The IMMULITE 2000 XPi Immunoassay System provides multiple tests on a single, easy-to-use analyzer, including the thyroid-stimulating immunoglobulin assay (Photo courtesy of Siemens Healthcare)

Immunoassays Evaluated for Thyroid-Stimulating Receptor Antibody in Graves’ Disease

Graves' disease (GD), also known as toxic diffuse goiter, is an autoimmune disease that affects the thyroid. It frequently results in and is the most common cause of hyperthyroidism and it also often results... Read more

Microbiology

view channel
Image: The sciREADER CL2 enables high quality digital colorimetric imaging of various support formats (Photo courtesy of SCIENION)

Multiplex Immunoassay Developed for Confirmation and Typing of HTLV Infections

Human T-Cell Lymphotropic Viruses (HTLV) type 1 and type 2 account for an estimated five to 10 million infections worldwide and are transmitted through breast feeding, sexual contacts and contaminated... Read more

Technology

view channel
Image: PKeye Workflow Monitor System (Photo courtesy of PerkinElmer, Inc.)

PerkinElmer’s New Cloud-Based Platform Enables Laboratory Personnel to Remotely Manage Its Instruments in Real-Time

PerkinElmer, Inc. (Waltham, MA; USA) has launched its PKeye Workflow Monitor, a cloud-based platform enabling laboratory personnel to remotely manage and monitor their PerkinElmer instruments and workflows... Read more

Industry

view channel
Illustration

Global Point of Care Diagnostics Market to Top USD 35 Billion by 2027 Due to Rising Diabetic Cases

The global point of care diagnostics market is projected to grow at a CAGR of close to 6% from more than USD 23 billion in 2020 to over USD 35 billion by 2027, driven by an increase in the number of diabetic... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.