We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Roche Diagnostics

Develops, manufactures, and markets a wide range of in vitro diagnostic systems, instruments, reagents, and tests read more Featured Products: More products

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Plasma-Based S100B Testing for Management of Traumatic Brain Injury

By LabMedica International staff writers
Posted on 24 May 2021
Print article
Image: The Elecys S100 Assay, an electro-chemiluminescence immunoassay (ECLIA) for the in vitro quantitative determination of S100 in human serum (Photo courtesy of Roche Diagnostics)
Image: The Elecys S100 Assay, an electro-chemiluminescence immunoassay (ECLIA) for the in vitro quantitative determination of S100 in human serum (Photo courtesy of Roche Diagnostics)
Traumatic brain injury (TBI) usually results from a violent blow or jolt to the head or body. An object that penetrates brain tissue, such as a bullet or shattered piece of skull, also can cause traumatic brain injury. Mild traumatic brain injury may affect the brain cells temporarily.

Serum biomarker S100B has been explored for its potential benefit to improve clinical decision-making in the management of patients suffering from TBI, especially as a pre-head computed-tomography screening test for patients with mild TBI. Although being already included into some guidelines, its implementation into standard care is still lacking.

Clinical Laboratorians at the University Medicine Mannheim (Mannheim, Germany) and their colleagues conducted retrospective study and compared, serum and lithium heparin blood samples from a total of 136 patients were analyzed for S100B concentrations. Specifically, 85 blood samples of stage I to IV melanoma patients were obtained during regular follow-up visits and additionally, they enrolled 51 consecutive patients presenting with TBI.

Serum samples were collected for the determination of S100B when clinically indicated together with lithium heparin blood for analysis of standard clinical chemistry analytes. S100B concentrations were determined using the Elecys S100 (Roche Diagnostics, Mannheim, Germany) assay on a Roche Cobas e411 instrument. The test is specified by the manufacturer for serum samples with a cut-off of 0.105 ng/mL and a linearity range of 0.005 – 39 ng/mL. Both S100B plasma and serum levels were tested for potential confounding variables.

The scientists reported that overall, S100B levels between both blood constituents correlated very well. The suitability of S100B testing from plasma was verified according to ISO15189 requirements. Using a cut-off of 0.105ng/ml, a sensitivity and negative predictive value of 100% were obtained for identifying patients with pathologic CT scans. Importantly, plasma-based testing reduced the turnaround time (TAT) to 26 minutes allowing for quicker clinical decision-making. The clinical utility of integrating S100B in TBI management was highlighted by two case reports.

The authors concluded that their study demonstrated the commutability of serum- and heparin-plasma-based S100B testing for TBI patients requiring rapid exclusion of organic brain damage. The analytical reliability of S100B testing from plasma was further proved by verification studies. The study was published on May 12, 2021 in the journal Practical Laboratory Medicine.

Related Links:
University Medicine Mannheim
Roche Diagnostics


Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
Complement 3 (C3) Test
GPP-100 C3 Kit
Gold Member
Xylazine Immunoassay Test
Xylazine ELISA

Print article

Channels

Molecular Diagnostics

view channel
Image: MOF materials efficiently enrich cfDNA and cfRNA in blood through simple operational process (Photo courtesy of Science China Press)

Blood Circulating Nucleic Acid Enrichment Technique Enables Non-Invasive Liver Cancer Diagnosis

The ability to diagnose diseases early can significantly enhance the effectiveness of clinical treatments and improve survival rates. One promising approach for non-invasive early diagnosis is the use... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.