We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
GLOBETECH PUBLISHING LLC

BECKMAN COULTER, INC.

Beckman Coulter develops, manufactures and markets laboratory systems, reagents, centrifugation, lab automation, elec... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
04 May 2021 - 07 May 2021
Virtual Venue

Basophil Activation Test Predicts Pediatric Wheezing Prognosis

By LabMedica International staff writers
Posted on 20 Jan 2021
Print article
Image: The CytoFLEX benchtop flow cytometer features up to three lasers for 13-color flow cytometry (Photo courtesy of Beckman Coulter Life Sciences).
Image: The CytoFLEX benchtop flow cytometer features up to three lasers for 13-color flow cytometry (Photo courtesy of Beckman Coulter Life Sciences).
Although asthma is a common chronic airway inflammatory disease in children, the natural history of asthma remains unknown. It is difficult to diagnose asthma in children aged under five years because of the complexity of wheezing phenotypes at that age.

Basophils are important immune cells that are involved in allergic diseases including asthma. CD63-based basophil activation tests (BATs) are a useful tool for not only identifying specific allergens, but also monitoring immunologic homeostasis. BATs are used for diagnosing food and drug allergies with high specificity and sensitivity.

A team of Pediatricians at the Shanghai Jiao Tong University School of Medicine (Shanghai, China) conducted a prospective cohort observation trial from July 2017 to March 2018 and enrolled children aged under five years with a convincing history of wheezing episodes (confirmed by the team or documented as “expiratory wheeze” in previous medical records). All enrolled subjects participated in specific immunoglobulin E (sIgE) testing. Serum samples were measured via the DX-Blot 45 Automatic Western Blotting instrument (Hangzhou Zhejiang Dixun Biological Genetic Engineering Co., Ltd. Hangzhou, Zhejiang, China).

The scientists collected anti-coagulated peripheral blood and stored at 2–8 °C for up to 6 hours. The Basophil Activation Test Set (Bühlmann Laboratories, Schönenbuch, Switzerland) was used for the BAT. In vitro stimulation was performed using an inhalant mix (Bühlmann Laboratories) that included common inhalation allergens (GX1, G12, T3, T4, W6, W9, M6, D1, D2, E1, and E2) as specific antigens. After processing and the red blood cells were lysed, the cells were resuspended in phosphate-buffered saline and assessed with a CytoFLEX flow cytometer (Beckman Coulter, Brea, CA, USA). The test result was considered positive when the level of CD63 expression in stimulated cells was >15% over the baseline level.

The investigators reported that of the 45 originally enrolled children, 38 completed both the follow-up and a BAT. After stimulation with the inhalant mixture, the CD63 expression on basophils and the rate of positive CD63-based BAT results in children diagnosed with asthma were both significantly higher than those in children who were not diagnosed with asthma. For the prediction of asthma, the positive predictive value and negative predictive value of CD63-based BAT was 71.8 and 69.2%, respectively. The positive likelihood ratio and negative likelihood ratio of CD63-based BAT were 1.70 and 0.3, respectively.

The authors concluded that their study indicates that CD63-based BAT has potential clinical value for predicting asthma outcome in young children with wheezing episodes. The study was published on January 6, 2021 in the journal BMC Immunology.

Related Links:
Shanghai Jiao Tong University School of Medicine
Hangzhou Zhejiang Dixun Biological Genetic Engineering Co
Bühlmann Laboratories
Beckman Coulter


Gold Supplier
Sample Transport
VACUETTE Transport Line
New
Silver Supplier
COVID-19 Ag Rapid Test
COVID-19 Ag Rapid Test Kit
New
SARS-CoV-2 PCR Assay
AppliedBiosystems TaqCheck SARS-CoV-2 Fast PCR Assay Kit
New
Gold Supplier
SARS-CoV-2 Antibody Test
SARS-CoV-2 UTAB FS

Print article
BIOHIT  Healthcare OY

Channels

Molecular Diagnostics

view channel
Image: The EZ DNA Methylation-Direct Kit (Photo courtesy of Zymo Research)

Leukocyte Epigenomics and Artificial Intelligence Predict Late-Onset Alzheimer’s Disease

Alzheimer’s Disease (AD) is the most common form of age-related dementia, accounting for 60%–80% of such cases. The disorder causes a wide range of significant mental and physical disabilities, with profound... Read more

Industry

view channel
Image: The Novodiag platform combines real-time PCR and microarray capabilities to provide high-level multiplexing (Photo courtesy of Mobidiag Ltd.)

Hologic to Acquire Mobidiag to Strengthen Diagnostic Testing Business

Hologic, Inc. (Marlborough, MA, USA) has signed a definitive agreement to acquire Mobidiag Ltd. (Espoo, Finland) in a USD 795 million transaction that will accelerate the global growth of Mobidiag’s differentiated... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.