We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Vaginal Bacteria Linked to Cervical Cancer

By LabMedica International staff writers
Posted on 27 Jun 2018
Print article
Image: The QuantStudio6 Flex real-time PCR system (Photo courtesy of Thermo Fisher Scientific).
Image: The QuantStudio6 Flex real-time PCR system (Photo courtesy of Thermo Fisher Scientific).
High-risk human papillomavirus (HPV) infection is a well-established risk factor for cervical cancer, but there are likely other factors within the local microenvironment that contribute to cervical carcinogenesis.

In some women, the vaginal microbiota (VMB) lacks a high proportion of lactobacilli and is dominated by a diverse mixture of anaerobic and microaerophilic bacteria, such as Gardnerella, Atopobium, Prevotella, Sneathia, commonly associated with bacterial vaginosis (BV).

Scientists at the University of Arizona (Phoenix, AZ, USA) recruited "100 premenopausal women" with "low- and high-grade" cervical abnormalities, invasive cervical cancer, and "healthy controls." The latter did not have any cervical abnormalities or cancer and included women with and without HPV. Vaginal swabs and cervicovaginal lavages (CVL) were collected by a clinician.

DNA was extracted from vaginal swabs using PowerSoil DNA Isolation Kit. HPV genotyping was performed on DNA samples extracted from vaginal swabs using the Linear Array HPV Genotyping Tests. Levels of 21 cytokines and chemokines were determined in CVL aliquots using the Milliplex MAP Human Cytokine/Chemokine and Th17 Magnetic Bead Panels. Level of Interleukin-36γ (IL-36γ) was measured in CVL aliquots by enzyme-linked immunosorbent assay using Human IL-36γ ELISA kit. The relative abundance of four Lactobacillus spp. was determined by quantitative real-time PCR analysis, performed on an Applied Biosystems QuantStudio6 Flex Real Time PCR System.

The authors reported that vaginal pH was associated with ethnicity and severity of cervical neoplasm. Lactobacillus dominance decreased with the severity of cervical neoplasm, which correlated with elevated vaginal pH. Hispanic ethnicity was also associated with decreased Lactobacillus dominance. Furthermore, Sneathia was enriched in all precancerous groups, invasive cervical carcinoma (ICC), abnormal pH and Hispanic origin. Notably, IL-36γ was significantly associated with ICC.

The authors concluded that local, host immune and microbial signatures associated with cervical carcinogenesis and provide an initial step to understanding the complex interplay between mucosal inflammation,and HPV persistence and the VMB. The study was published on May 15, 2018, in the journal Scientific Reports.

Related Links:
University of Arizona

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
POCT Fluorescent Immunoassay Analyzer
FIA Go
Gold Member
Real-time PCR System
GentierX3 Series

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: MOF materials efficiently enrich cfDNA and cfRNA in blood through simple operational process (Photo courtesy of Science China Press)

Blood Circulating Nucleic Acid Enrichment Technique Enables Non-Invasive Liver Cancer Diagnosis

The ability to diagnose diseases early can significantly enhance the effectiveness of clinical treatments and improve survival rates. One promising approach for non-invasive early diagnosis is the use... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.