We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
INTEGRA BIOSCIENCES AG

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Protein Biomarker Found for Early-Stage Lung Cancer

By LabMedica International staff writers
Posted on 31 May 2018
Print article
Image: The Genepix 4000B microarray scanner (Photo courtesy of Molecular Devices).
Image: The Genepix 4000B microarray scanner (Photo courtesy of Molecular Devices).
The American Cancer Society (ACS) estimate that in 2018, 154,050 people in the USA will have died from lung cancer and the World Health Organization (WHO) suggest that 1.69 million deaths are brought about by lung cancer worldwide.

Most lung cancers are initially diagnosed at an advanced stage, and so the disease is associated with a poor prognosis, being the leading cause of cancer-related death worldwide. The identification of patients at a resectable early stage of cancer is therefore extremely important.

Scientists at the Kitasato University School of Allied Health Sciences (Kanagawa, Japan) obtained frozen fresh lung cancer tissues and kept at 80 °C until use for proteomic analysis. Sera from 271 patients with lung cancer and 100 healthy controls were used as the training set. In addition, a validation set consisting of sera from 100 patients with lung cancer and 38 healthy controls was also studied.

The team developed monoclonal antibodies to validate their studies. They also performed immunoblotting and the immunoreactive bands on the membranes were detected with Immobilon Western Chemiluminescent HRP Substrate and captured with ATTO Cool Saver System. The team also carried out immunohistochemical staining and for reverse-phase protein array (RPPA) the stained slides were scanned on a microarray scanner Genepix 4000B.

Of the monoclonal antibodies generated, one antibody designated as KU-Lu-1, recognized cytoskeleton-associated protein 4 (CKAP4). CKAP4 was detected in lung cancer cells and tissues, and its secretion into the culture supernatant was also confirmed. The serum CKAP4 levels of lung cancer patients were significantly higher than those of healthy controls. Furthermore, the serum CKAP4 levels were also higher in patients with stage I adenocarcinoma or squamous cell carcinoma than in healthy controls. Serum CKAP4 levels may differentiate lung cancer patients from healthy controls, and they may be detected early even in stage I non–small cell lung cancer. Serum CKAP4 levels were also significantly higher in lung cancer patients than in healthy controls in the validation set.

Yuichi Sato, PhD, a professor of Molecular Diagnostic, and senior author of the study, said, “The use of CKAP4 as a biomarker could change current practices regarding the treatment of lung cancer patients, and the diagnostic accuracies may be markedly improved by the combination of CKAP4 and conventional markers.” The study was published on May 8, 2018, in The American Journal of Pathology.

Related Links:
Kitasato University School of Allied Health Sciences

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
POCT Fluorescent Immunoassay Analyzer
FIA Go
New
Gold Member
TORCH Panel Rapid Test
Rapid TORCH Panel Test

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: MOF materials efficiently enrich cfDNA and cfRNA in blood through simple operational process (Photo courtesy of Science China Press)

Blood Circulating Nucleic Acid Enrichment Technique Enables Non-Invasive Liver Cancer Diagnosis

The ability to diagnose diseases early can significantly enhance the effectiveness of clinical treatments and improve survival rates. One promising approach for non-invasive early diagnosis is the use... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.