We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

STANDARD DIAGNOSTICS INC.

Standard Diagnostics develops rapid diagnostic tests that make it possible to diagnose various diseases at an earlier... read more Featured Products: More products

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Prototype Rapid Diagnostic Test Detects Human African Trypanosomiasis

By LabMedica International staff writers
Posted on 27 Apr 2016
Print article
Image: The SD BIOLINE HAT prototype rapid diagnostic test for the detection of Trypanosoma brucei gambiense infection (Photo courtesy of Standard Diagnostics).
Image: The SD BIOLINE HAT prototype rapid diagnostic test for the detection of Trypanosoma brucei gambiense infection (Photo courtesy of Standard Diagnostics).
Early diagnosis and treatment of human African trypanosomiasis (HAT) is essential for safe and effective treatment but the tests used to screen suspected patients and populations at risk are difficult to implement in remote rural settings where the disease occurs.

The availability of simple, easy to use, instrument-free rapid diagnostic tests would improve screening and coverage of the population at risk and contribute to elimination of the disease. It would enable technicians with limited training and clinicians in emergency or medical wards to make rapid differential diagnosis for neurological syndromes or malaria-like illnesses.

Scientists at the Institute of Neuroepidemiology and Tropical Neurology (Limoges, France) and their African colleagues enrolled study participants from HAT endemic regions during both active and passive screening activities by teams of the national sleeping sickness control programs. Cases of HAT were defined as subjects in whom trypanosomes were demonstrated by microscopy in blood, lymph node aspirate or cerebral spinal fluid (CSF).

The test used in the study was the prototype SD BIOLINE HAT (Alere/Standard Diagnostics, Inc., Yongin-si, South Korea) which is an immunochromatographic test for qualitative detection of antibodies of all isotypes (immunoglobulin G (IgG), IgA and IgM). The rapid diagnostic test (RDT) has a nitrocellulose membrane strip with two regions (T1 and T2) that are pre-coated with two native variant surface glycoprotein (VSG) antigens from Trypanosoma brucei gambiense (VSG LiTat1.3 and VSG LiTat 1.5 respectively). It also has a procedural control line (C). The prototype RDTs were compared with the reference antibody detection screening test in current use, the card agglutination test for trypanosomiasis (CATT/T.b.gambiense).

In total, 14,818 participants were screened using both serological tests (9.5% in passive screening), and out of these 149 HAT cases were confirmed by parasitology. Among the 149 confirmed cases, 148 were positive by either CATT on whole blood and/or RDT, while one case was negative by both CATT whole blood and RDT, and was detected by CATT dilutions. The sensitivity and specificity of the prototype SD BIOLINE HAT was 89.3% and 94.6% respectively. The sensitivity and specificity of the CATT on whole blood were 94.0% and 95.9%, and of the CATT diluted 1/8 were 89.3% and 98.9% respectively.

The authors concluded that after further optimization, the prototype SD BIOLINE HAT could become an alternative to current screening methods in primary healthcare settings in remote, resource-limited regions where HAT typically occurs. The study was published on April 8, 2016, in the journal Public Library of Science Neglected Tropical Diseases.

Related Links:
Institute of Neuroepidemiology and Tropical Neurology
Standard Diagnostics
Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
Gold Member
ADAMTS-13 Protease Activity Test
ATS-13 Activity Assay

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: MOF materials efficiently enrich cfDNA and cfRNA in blood through simple operational process (Photo courtesy of Science China Press)

Blood Circulating Nucleic Acid Enrichment Technique Enables Non-Invasive Liver Cancer Diagnosis

The ability to diagnose diseases early can significantly enhance the effectiveness of clinical treatments and improve survival rates. One promising approach for non-invasive early diagnosis is the use... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.