We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
INTEGRA BIOSCIENCES AG

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Novel Chromogenic Screening Medium Developed for MRSA

By LabMedica International staff writers
Posted on 13 Aug 2014
Print article
Image: Typical colonies of methicillin-resistant Staphylococcus aureus (MRSA) grew and formed blue-colored colonies on MRSA-chrom media after 24 hours of incubation at 35 °C (Photo courtesy of Kohjin Bio Co., Ltd).
Image: Typical colonies of methicillin-resistant Staphylococcus aureus (MRSA) grew and formed blue-colored colonies on MRSA-chrom media after 24 hours of incubation at 35 °C (Photo courtesy of Kohjin Bio Co., Ltd).
A novel chromogenic screening agar medium for methicillin-resistant Staphylococcus aureus (MRSA) has been developed, as rapid and definite detection of MRSA is important not only diagnosis but also for preventing the spread of this bacteria.

MRSA is known as a major nosocomial pathogen, which causes a variety of clinical symptoms including septicemia, pneumonia, and postsurgical toxic shock syndrome and treatment of infections by MRSA has been problematic since MRSA is resistant to many antimicrobial agents.

Scientists at the Kohjin Bio Co., Ltd. (Saitama, Japan) compared the company’s MRSA-chrom media with other commercially available media to detect MRSA. Forty-two strains of MRSA including 41 clinical isolates from nasal cavity were inoculated into the media for the inclusivity study. For the exclusivity study, 52 gram-positive strains other than MRSA, 36 gram-negative strains, and 3 yeasts were inoculated into suitable culture media.

A total of 42 MRSA strains grew and formed typical blue- and mauve-colored colonies on MRSA-chrom and CHROMagar MRSA (CHROMagar Microbiology; Paris, France) in 24 hours. Of 42 MRSA strains, 37 strains grew and formed typical yellow colonies with egg yolk turbid reaction for S. aureus on three other media in 48 hours. All of the other microbes tested including 17 methicillin-sensitive S. aureus (MSSA) strains failed to grow on MRSA-chrom. The colonies of Bacillus cereus were spread and had a frosted glass appearance and as a result, these colonies could be distinguished from MRSA easily. When the scientists tested the 50 clinical specimens, they found that both chromogenic media detected MRSA as typical colored colony from 33 specimens after 24 hours of incubation.

The authors concluded that the novel chromogenic medium, MRSA-chrom, developed for the screening of MRSA was excellent for both selectivity and detectability. Therefore, they suggested that MRSA-chrom was a useful alternative for rapid and definitely screening of MRSA. Moreover, MRSA-chrom had a good growth for MRSA since it contained no sodium chloride. The study was published in the August 2014 issue of the journal Diagnostic Microbiology and Infectious Disease.

Related Links:

Kohjin Bio Co., Ltd.
CHROMagar Microbiology


Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
One Step HbA1c Measuring System
GREENCARE A1c
POCT Fluorescent Immunoassay Analyzer
FIA Go
New
Gold Member
Fully Automated Cell Density/Viability Analyzer
BioProfile FAST CDV

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: MOF materials efficiently enrich cfDNA and cfRNA in blood through simple operational process (Photo courtesy of Science China Press)

Blood Circulating Nucleic Acid Enrichment Technique Enables Non-Invasive Liver Cancer Diagnosis

The ability to diagnose diseases early can significantly enhance the effectiveness of clinical treatments and improve survival rates. One promising approach for non-invasive early diagnosis is the use... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.