Features | Partner Sites | Information | LinkXpress
Sign In
SIEMENS DIAGNOSTICS

Innovative Technique Diagnoses Intestinal Parasitic Infections

By Labmedica International staff writers
Posted on 12 Aug 2013
Image: The fill-FLOTAC and mini-FLOTAC Devices (Photo courtesy of San Raffaele Hospital).
Image: The fill-FLOTAC and mini-FLOTAC Devices (Photo courtesy of San Raffaele Hospital).
A recently developed method for directly diagnosing intestinal parasites has been evaluated in the field and compared with standard methodology.

Soil-transmitted helminthes and intestinal protozoa infection are widespread in developing countries, yet an accurate diagnosis is rarely performed as the highly trained laboratory scientists required are seldom available in poor resource settings.

Microbiologists at the San Raffaele Hospital (Milan, Italy) in collaboration with others, analyzed stool samples from 180 pupils from two primary schools in India and Tanzania. Three diagnostic methods were used: direct fecal smear, formol-ether concentration method (FECM) and mini-FLOTAC. The latter is a simplified device and one of the main advantages of this method is that it can be more easily transferred and carried out in laboratories with limited facilities as there is no centrifugation required. The mini-FLOTAC comprises two physical components, the base and the reading disc. There are two 1-mL flotation chambers, which are designed for optimal examination of fecal sample suspensions in each flotation chamber and which permits a maximum magnification of ×400.

Overall, 72% of the pupils were positive for any intestinal parasitic infection, 24% carried dual infections, and 11% had three infections or more. The most frequently encountered intestinal parasites were Entamoeba coli, Entamoeba histolytica/dispar, Giardia intestinalis, hookworm, and Schistosoma mansoni in Tanzania. Significant differences were found in the detection of parasitic infections among the three methods. The mini-FLOTAC was the most sensitive method for helminthic infections at 90%, for the FECM provided 60% detection, and only 30% for the direct fecal smear. However, the FECM was the most sensitive for intestinal protozoa infections at 88% with 70% for direct fecal smear, and 68% for the mini-FLOTAC.

The authors concluded that the mini-FLOTAC for the diagnosis of intestinal helminthes and protozoa is a valid, sensitive, and potentially low-cost alternative technique that could be used in resource-limited settings, particularly for helminthic diagnosis. The mini-FLOTAC and fill-FLOTAC were invented and patented by Prof. Giuseppe Cringoli (University of Naples Federico II, Italy).

Related Links:

San Raffaele Hospital
University of Naples Federico II



comments powered by Disqus

Channels

Immunology

view channel
Image: The fluorescence-activated cell sorting FACSCanto II flow cytometer (Photo courtesy of BD Biosystems).

Flow Cytometry Detects Lymphoproliferative Disorders in Fluid Specimens

Immunophenotypic analysis of hematopoietic cell populations by flow cytometry has emerged as a useful ancillary study in the diagnostic evaluation of serous effusions and cerebrospinal fluids (CSFs).... Read more

Pathology

view channel
Image: Histopathology of increased endomysial connective tissue from a patient with Duchenne Muscular Dystrophy. Necrotic fibers arrowed (Photo courtesy of Washington University in St. Louis).

New Partnership Designed to Automate Dystrophin Measurement

A developer of innovative ribonucleic acid (RNA)-based therapeutics, Sarepta Therapeutics, Inc. ( Cambridge, MA, USA), and a leading tissue-based companion diagnostics firm, Flagship Biosciences LLC (Boulder,... Read more

Lab Tech.

view channel
Image: A technical breakthrough for DNA imaging has been achieved that should quicken diagnosis of diseases for which analysis of DNA from single-cells is critical, such as early stage cancers and various pre-natal conditions (Photo courtesy of McGill University and Génome Québec Innovation Center).

Breakthrough DNA Analysis Technology to Hasten Problem Diagnosis

Researchers have achieved a technical breakthrough that should result in speedier diagnosis of diseases for which analysis of DNA from single-cells is critical, such as early stage cancers and various... Read more

Industry News

view channel

Most IVD Sector Companies Look Outside the EU and USA for Growth

Kalorama Information (New York City, NY, USA) has now published the 9th edition of its biennial market research report “Worldwide Market for In Vitro Diagnostics,” revealing estimates from its survey of the in vitro diagnostics (IVD) industry – presenting the trends, technologies, customer needs, and major suppliers with... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.