Features | Partner Sites | Information | LinkXpress
Sign In
FOCUS DIAGNOSTICS, INC.
AB Sciex
Bio-Rad Diabetes Division

Innovative Technique Diagnoses Intestinal Parasitic Infections

By Labmedica International staff writers
Posted on 12 Aug 2013
Image: The fill-FLOTAC and mini-FLOTAC Devices (Photo courtesy of San Raffaele Hospital).
Image: The fill-FLOTAC and mini-FLOTAC Devices (Photo courtesy of San Raffaele Hospital).
A recently developed method for directly diagnosing intestinal parasites has been evaluated in the field and compared with standard methodology.

Soil-transmitted helminthes and intestinal protozoa infection are widespread in developing countries, yet an accurate diagnosis is rarely performed as the highly trained laboratory scientists required are seldom available in poor resource settings.

Microbiologists at the San Raffaele Hospital (Milan, Italy) in collaboration with others, analyzed stool samples from 180 pupils from two primary schools in India and Tanzania. Three diagnostic methods were used: direct fecal smear, formol-ether concentration method (FECM) and mini-FLOTAC. The latter is a simplified device and one of the main advantages of this method is that it can be more easily transferred and carried out in laboratories with limited facilities as there is no centrifugation required. The mini-FLOTAC comprises two physical components, the base and the reading disc. There are two 1-mL flotation chambers, which are designed for optimal examination of fecal sample suspensions in each flotation chamber and which permits a maximum magnification of ×400.

Overall, 72% of the pupils were positive for any intestinal parasitic infection, 24% carried dual infections, and 11% had three infections or more. The most frequently encountered intestinal parasites were Entamoeba coli, Entamoeba histolytica/dispar, Giardia intestinalis, hookworm, and Schistosoma mansoni in Tanzania. Significant differences were found in the detection of parasitic infections among the three methods. The mini-FLOTAC was the most sensitive method for helminthic infections at 90%, for the FECM provided 60% detection, and only 30% for the direct fecal smear. However, the FECM was the most sensitive for intestinal protozoa infections at 88% with 70% for direct fecal smear, and 68% for the mini-FLOTAC.

The authors concluded that the mini-FLOTAC for the diagnosis of intestinal helminthes and protozoa is a valid, sensitive, and potentially low-cost alternative technique that could be used in resource-limited settings, particularly for helminthic diagnosis. The mini-FLOTAC and fill-FLOTAC were invented and patented by Prof. Giuseppe Cringoli (University of Naples Federico II, Italy).

Related Links:

San Raffaele Hospital
University of Naples Federico II



DiagCor Bioscience
77 ELEKTRONIKA
PURITAN MEDICAL
comments powered by Disqus
Life Technologies

Channels

Clinical Chemistry

view channel
Image: The Cobas 6000 Multipurpose Clinical Laboratory analyzer (Photo courtesy of Roche Diagnostics).

Low Density Lipoprotein Apolipoprotein-B Assay Validated

The relationship between apolipoprotein-B (Apo-B) concentration and the risk of developing cardiovascular disease (CVD) is more robust than the relationship between low density lipoprotein cholesterol... Read more

Genetic Tests

view channel
Histopathology of colonic adenocarcinoma; the glands are enlarged and filled with necrotic debris (Photo courtesy of the University of Utah).

Promising Blood Biomarkers Isolated for Colorectal Cancer

The search for blood-borne biomarkers that could be used to screen for colorectal cancer (CRC) has uncovered two promising candidates that may one day lead to the development of a simple blood test.... Read more

Industry News

view channel

Collaboration to Bring Personalized Cancer Care to Singapore

A German company specializing in cancer diagnosis will be extending its expertise in personalized care to the health care market in Singapore. New Oncology (Cologne, Germany) announced recently that it has entered into a collaboration agreement with the National Cancer Centre Singapore (Singapore). New Oncology will... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.