Features | Partner Sites | Information | LinkXpress
Sign In
FOCUS DIAGNOSTICS, INC.
GLOBETECH PUBLISHING LLC
RANDOX LABORATORIES

Biomolecular System Launched for Proteins Quantitation

By Labmedica International staff writers
Posted on 02 Apr 2012
Image: Direct Detect Protein Quantitation System (Photo Courtesy of EMD Millipore).
Image: Direct Detect Protein Quantitation System (Photo Courtesy of EMD Millipore).
A system for rapid, simplified protein quantitation has been introduced that enables infrared-based measurement of amide bonds in protein chains.

The system does not rely on amino acid composition, dye-binding properties, or redox potential unlike conventional assays based on ultraviolet- visual (UV-Vis) spectroscopy, which do rely on absorbance by a protein’s aromatic amino acids and therefore have limited utility.

The Direct Detect system employs a hydrophilic polytetrafluoroethylene (PTFE) membrane designed to be transparent in most of the infrared spectral region and enables application of biomolecular solutions directly onto the membrane. The system delivers more universally applicable and faster protein quantitation, requiring minimal sample consumption. System accuracy and precision are comparable with results obtained by amino acid analysis, an accurate standard for quantitation, yet can be time-consuming and often costly.

The system relies on infrared-based detection of biomolecules, so that the users can obtain accurate and reproducible protein quantitation in the presence of reducing agents and detergents. As a result, the Direct Detect (EMD Millipore, Billerica, MA, USA) system can measure protein concentrations from 0.2 mg/mL to 5 mg/mL within seconds, without any bio- or immunochemical staining, directly from samples, including buffered solutions.

John Sweeney, Head of Life Science Business Field, EMD Millipore, said, "In addition to its analytical powers, the Direct Detect system provides a groundbreaking departure from traditional sample preparation requirements typical of biomolecular quantitation. After samples are spotted on assay cards, they can be stored in ambient conditions without appreciable change in readout. Another important advantage over conventional assays is that the Direct Detect calibration standard curve requires generation once, which provides additional time savings and ease of use." EMD Millipore is a division of Merck (Darmstadt, Germany).

Related Links:

EMD Millipore
Merck



MEDLAB Asia
CELLAVISION AB
KARL HECHT GMBH & CO KG
PERIPHERAL VISIONS INC

Channels

Clinical Chemistry

view channel
Image: UniCel DxC 800 Synchron Clinical Systems (Photo courtesy of Beckman Coulter).

Routine Blood Glucose Value Correlates with Diabetes Risk

Random glucose values obtained during routine blood tests are often overlooked, but could provide valuable insight into whether someone is at risk for having type 2 diabetes. Random Blood Glucose (RBG)... Read more

Genetic Tests

view channel
Image: Histopathology of tuberculoid leprosy in a skin section (Photo courtesy Dr. D.S. Ridley, Wellcome Images).

Genes Discovered Influence Risk of Developing Leprosy

Leprosy, a chronic dermatological and neurological disease, is caused by infection with Mycobacterium leprae, and its manifestation, progression and prognosis are strongly associated with the proficiency... Read more

Hematology

view channel
Image: Plastic bag containing 0.5 to 0.7 liters of packed red blood cells in citrate, phosphate, dextrose, and adenine (CPDA) solution (Photo courtesy of Fresenius HemoCare).

Transfusion Protocols Compared After Cardiac Surgery

Unnecessary blood transfusions may increase healthcare costs both directly, because blood is an increasingly scarce and expensive resource, and indirectly due to the complications associated with transfusion.... Read more

Industry News

view channel

Sequencing-Based Testing Sector Already Highly Competitive

As next-generation sequencing (NGS) reaches the clinical laboratory, a new analysis by Kalorama Information (New York, NY, USA) finds that small reference laboratories as well as over 50 companies now offer sequencing-based testing through their own CLIA-certified laboratories, some of which offer a wide range of tests.... Read more
 

Events

28 Mar 2015 - 01 Apr 2015
06 Apr 2015 - 08 Apr 2015
07 Apr 2015 - 09 Apr 2015
Copyright © 2000-2015 Globetech Media. All rights reserved.