Features | Partner Sites | Information | LinkXpress
Sign In
Bio-Rad Diabetes Division
RANDOX LABORATORIES
PURITAN MEDICAL

Biomolecular System Launched for Proteins Quantitation

By Labmedica International staff writers
Posted on 02 Apr 2012
Image: Direct Detect Protein Quantitation System (Photo Courtesy of EMD Millipore).
Image: Direct Detect Protein Quantitation System (Photo Courtesy of EMD Millipore).
A system for rapid, simplified protein quantitation has been introduced that enables infrared-based measurement of amide bonds in protein chains.

The system does not rely on amino acid composition, dye-binding properties, or redox potential unlike conventional assays based on ultraviolet- visual (UV-Vis) spectroscopy, which do rely on absorbance by a protein’s aromatic amino acids and therefore have limited utility.

The Direct Detect system employs a hydrophilic polytetrafluoroethylene (PTFE) membrane designed to be transparent in most of the infrared spectral region and enables application of biomolecular solutions directly onto the membrane. The system delivers more universally applicable and faster protein quantitation, requiring minimal sample consumption. System accuracy and precision are comparable with results obtained by amino acid analysis, an accurate standard for quantitation, yet can be time-consuming and often costly.

The system relies on infrared-based detection of biomolecules, so that the users can obtain accurate and reproducible protein quantitation in the presence of reducing agents and detergents. As a result, the Direct Detect (EMD Millipore, Billerica, MA, USA) system can measure protein concentrations from 0.2 mg/mL to 5 mg/mL within seconds, without any bio- or immunochemical staining, directly from samples, including buffered solutions.

John Sweeney, Head of Life Science Business Field, EMD Millipore, said, "In addition to its analytical powers, the Direct Detect system provides a groundbreaking departure from traditional sample preparation requirements typical of biomolecular quantitation. After samples are spotted on assay cards, they can be stored in ambient conditions without appreciable change in readout. Another important advantage over conventional assays is that the Direct Detect calibration standard curve requires generation once, which provides additional time savings and ease of use." EMD Millipore is a division of Merck (Darmstadt, Germany).

Related Links:

EMD Millipore
Merck



DIASYS DIAGNOSTIC SYSTEMS
KARL HECHT GMBH & CO KG
ADVANCED INSTRUMENTS
WATERS CORPORATION

Channels

Genetic Tests

view channel
Image: Histopathology of pancreatic adenocarcinoma arising in the pancreas head region (Photo courtesy of Dr. KGH/Wikimedia).

Multiple Genetic Changes Linked to Increased Pancreatic Cancer Risk

A genome-wide association study, believed to be the largest of its kind, has uncovered four regions in the human genome where changes may increase the risk of pancreatic cancer. Patients with pancreatic... Read more

Immunology

view channel

Powerful Innovations in Diagnostics Unveiled at EuroMedLab 2015

New systems presented by Roche include a new standard for immunology testing, an integrated platform for hematology diagnostics, and a high-throughput analyzer to perform HbA1c tests for blood glucose management. Also, evidence was presented for a high-sensitivity cardiac troponin test to provide earlier and more accurate... Read more

Industry News

view channel

Next Generation Sequencing Provides Responsiveness for Identifying Resistant Pathogens

According to a new market analysis, next generation sequencing (NGS) may impact the infectious diseases IVD sector by bypassing some of the downside of PCR-based testing, as NGS can provide more flexibility and responsiveness in identification of new pathogenic strains. NGS may earn its place in clinical testing as its... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.