Features | Partner Sites | Information | LinkXpress
Sign In
PURITAN MEDICAL
Biostrata Ltd on behalf of Thermo
FOCUS DIAGNOSTICS, INC.

NIST Standard Will Help Diagnosis, Treatment of Cytomegalovirus

By Labmedica International staff writers
Posted on 23 Jan 2012
A new clinical Standard Reference Material (SRM) will help healthcare professionals more accurately diagnose and treat Cytomegalovirus (CMV), a common pathogen that is particularly dangerous for infants and persons with weakened immune systems.

If a CMV infection becomes dangerous, antiviral agents can be used to moderate the impact. Many of these compounds are toxic, so the physician must know the severity of the infection--a measure known as viral load (number of virus copies per microliter of blood)--to prescribe the optimal dosage and duration of treatment.

The current means of measuring viral load is to use polymerase chain reaction (PCR) to amplify a region of the CMV gene and then use a calibration curve to estimate the number of virus particles in the original sample. Accuracy of these measurements can vary greatly from one test facility to another, as there are many different PCR protocols used to determine viral load, including commercial and "in-house" laboratory assays.

The new [US] National Institute of Standards and Technology (NIST; Gaithersburg, MD, USA) reference, SRM 2366, provides a standardized CMV DNA. Consistency of the viral DNA in the standard was ensured by manufacturing it in Escherichia coli bacteria. These E. coli cells each contain a copy of the CMV genome in a "DNA construct"--an artificially constructed segment of nucleic acid that codes for a specific product, in this case, CMV DNA. The DNA copies made by this E. coli cell culture can then be purified and quantified using digital polymerase chain reaction (PCR).

SRM 2366 consists of three solutions, each with a specific concentration of CMV DNA copies per microliter: 420, 1,702, and 19,641. These are designed to qualify prepared calibration samples. They also can be used as quality control samples for diagnostic equipment. For added traceability, the SRM certificate of analysis includes the genetic sequences of the nine CMV genome regions copied for the standard.

CMV is found in 50% to 80 % of the population. CMV generally remains latent in an infected person unless certain conditions trigger its activation. CMV poses a significant health risk to people who are immunocompromised and babies who receive the virus from their mothers before birth. Congenital CMV infections cause more long-term problems and childhood deaths than many other prenatal disorders including fetal alcohol syndrome, Down syndrome, and neural tube defects such as spina bifida.

Related Links:

National Institute of Standards and Technology




KARL HECHT GMBH & CO KG
77 ELEKTRONIKA
CELLAVISION AB

Channels

Genetic Tests

view channel
Image: Histopathology of a coronary artery with the most common form of obstructive coronary artery disease (atherosclerosis) and marked luminal narrowing (Photo courtesy of Nephron).

Blood Test Influences Clinical Decisions in Coronary Artery Disease

Clinicians need better approaches to evaluating women at midlife and beyond who present to primary care with chest pain and related symptoms such as an individual's current likelihood of having obstructive... Read more

Microbiology

view channel
Image: The VIKIA Malaria Ag Pf/Pan rapid diagnostic test (Photo courtesy of IMACCESS).

Malaria Rapid Diagnostic Test Evaluated At Point-Of-Care

For over a decade, the development of malaria rapid diagnostic tests (RDTs) has enabled reliable biological diagnostic testing in all situations where previously only clinical diagnosis was available.... Read more

Industry News

view channel

Clinical Lab Automation Sector at USD 5.4 Billion and Growing

Lab automation systems sold to clinical laboratories reached USD 5.4 billion in sales in 2014. Shortage of personnel will further drive these purchases, as will a need for new systems, according to a new market research report on the status of lab automation in both clinical and drug-discovery lab segments.... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.