Features | Partner Sites | Information | LinkXpress
Sign In
RANDOX LABORATORIES
FOCUS DIAGNOSTICS, INC.
Biostrata Ltd on behalf of Thermo

Expression of Antigen Evaluated for Prostate Cancer

By Labmedica International staff writers
Posted on 14 Feb 2012
The expression of the cancer/testis antigen, A-kinase anchor protein 4 (AKAP-4), has been evaluated in prostate cancer (PC) patients.

The exploitation tumor-associated antigens, not only for screening purposes, but also as targets for tumor immunotherapy underlines their relevance in oncology and is due to features frequently shared by molecular biomarkers and tumor antigens.

Scientists at Texas Technical University (Lubbock, TX, USA) analyzed normal prostate tissues, 15 patients with PC and the LnCAP PC cell line by immunohistochemistry. The immunogenicity of AKAP-4 was tested through indirect enzyme-linked immunosorbent assay (ELISA) on sera from cancer patients and healthy subjects. In vitro AKAP-4-specific cytotoxic lymphocytes were generated from peripheral blood mononuclear cells. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to measure AKAP-4 messenger ribonucleic acid (mRNA) expression.

The AKAP-4 was shown both at the cytoplasmic and surface levels of the LnCAP PC cell line. AKAP-4 was also highly expressed in PC cells from patients. Specific anti-AKAP-4 circulating immunoglobulins were detected in AKAP-4 positive subjects. The use of recombinant AKAP-4-loaded autologous dendritic cells generated AKAP-4-specific and human leukocyte antigen-1 (HLA-I)-restricted cytotoxic T lymphocytes able to kill PC cells in vitro. Further characterization indicated a T-helper cell (Th-1) skewing in the cytokine secretion profile of these cells.

The indirect ELISA was performed using specific reagents from Pierce (Rockford, IL, USA) and the ELISA for T Cell Cytokine Expression used sandwich ELISA kits from U-CyTech (Utrecht, The Netherlands). The authors concluded that the aberrant expression of AKAP-4 in PC will potentially be developed as a biomarker in PC and is a potential target for immunotherapy. The study was published in the January 2012 issue of the journal the Prostate.

Related Links:

Texas Technical University
Pierce
U-CyTech



KARL HECHT GMBH & CO KG
DIASYS DIAGNOSTIC SYSTEMS
EUROIMMUN AG
WATERS CORPORATION

Channels

Genetic Tests

view channel
Image: Histopathology of a spinal cord from an infant with multiple joint contractures present at birth (Photo courtesy of Dimitri P. Agamanolis, MD).

Infant Stiff-Joint Syndromes Matched to Possible Genetic Origins

There are over 400 specific disorders that are, or can be, associated with multiple contractures in the newborn and a better understanding of disorders with contractures and their associated genetics is... Read more

Pathology

view channel

Panel of Bloodstream MicroRNAs Predicts Damage from Radiation Exposure

A panel of microRNAs (miRNAs) that can be measured in blood samples is able to predict the extent of long-term radiation injury and likelihood of survival following exposure to high doses of radiation. Accidental radiation exposure is a threat to human health that necessitates effective clinical planning and diagnosis.... Read more

Industry News

view channel

Main Factors Driving Revenue Growth in Clinical Chemistry Identified

According to a report by Kalorama Information (New York, NY, USA), overall growth in the clinical chemistry market will be modest, driven mainly by new tests and demographic changes, including an aging population and increasing number of people at risk of cardiovascular disease, such as those with hypertension, diabetes... Read more
 

Events

27 May 2015 - 29 May 2015
02 Jun 2015 - 03 Jun 2015
06 Jun 2015 - 09 Jun 2015
Copyright © 2000-2015 Globetech Media. All rights reserved.