Features | Partner Sites | Information | LinkXpress
Sign In
FOCUS DIAGNOSTICS, INC.
RANDOX LABORATORIES
PURITAN MEDICAL

Blood Test Diagnoses Cause of Kidney Failure

By Labmedica International staff writers
Posted on 01 Feb 2012
Discoveries about the basic workings of endothelial cells could lead to a diagnostic test for the serious kidney disease known as hemolytic uremic syndrome (HUS).

HUS is a potentially life-threatening condition and often occurs after gastrointestinal infection with Escherichia coli O157:H7, which produces Shiga toxins (Stx) that cause hemolytic anemia, thrombocytopenia, and renal injury.

Scientists at University of Toronto (ON, Canada) discovered a biological pathway never before known to have played a role in the development of HUS. Specifically, they found that the toxin can increase the level of a chemokine, namely stromal cell-derived factor-1 (SDF-1), and its receptor, C-X-C chemokine receptor type 4 (CXCR4). Chemokines are small secreted proteins that stimulate cells to move or migrate. CXCR4 was already known to stimulate the release and migration of the precursors of white blood cells from bone marrow, to change how blood vessels grow and to help the AIDS virus enter cells.

Blood tests were performed on children with E. coli and all measurements of SDF-1 were performed on blinded samples by a sandwich enzyme-linked immunosorbent assay (ELISA) (R&D Systems, Minneapolis, MN, USA). The results indicated that those who went on to develop HUS had as much as four times higher levels of the protein SDF-1, than other children with E. coli who did not go on to develop HUS. This suggests that a blood test could be used to predict who is most likely to develop the potentially fatal HUS, meaning they could be monitored more closely. The scientists found that too much communication between SDF-1 and CXCR4 molecules can also impact the development of HUS in animals and humans.

Philip A. Marsden, MD, FRCPC, who is a nephrologist at Saint Michael’s Hospital (Toronto, ON, Canada) and senior author of the study, said, "A safe water supply and clean food supply chain is the most important step in preventing HUS caused by E. coli. If we can measure SDF-1 levels in real time during an E. coli outbreak and confirm these findings, then we have a strong case for a trial of CXCR4 antagonists in patients with toxin-producing E. coli to see if it prevents or improves cases of HUS.” The study was published on January 9, 2012, in the Journal of Clinical Investigation.

Related Links:
University of Toronto
R&D Systems
Saint Michael’s Hospital


DIASYS DIAGNOSTIC SYSTEMS
KARL HECHT GMBH & CO KG
Dubai Bio Expo-2015

Channels

Genetic Tests

view channel
Image: The final result of a DNA sequencing process, with each color representing one of the four base chemicals, adenine, guanine, cytosine and thymine, that comprise DNA (Photo courtesy of Gerald Barber).

Gene Sequencing Reference Standard Increases Accuracy for Medical Laboratories

To reduce the variability in genetic test results that has been observed across different clinical laboratories and pathology groups, a new standard DNA reference has been introduced, which will improve... Read more

Hematology

view channel
Image: Immunochemistry of bone marrow biopsy from an acute myeloid leukemia patient showing sheets of CD34 positive cells; corresponding bone marrow aspirate showed only 20% blasts (Photo courtesy of All India Institute of Medical Sciences).

Immunohistochemistry Assesses Early Response in Acute Myeloid Leukemia

Acute myeloid leukemia (AML) is a heterogeneous disease with respect to prognosis and early response assessment has an established role as predictor of remission rate, and overall and disease-free survival.... Read more

Industry News

view channel

Partners to Develop and Commercialize Noninvasive Tests for Bladder Cancer

An agreement between a major European university medical center and a multinational biotechnology company was designed to promote commercialization and further development of a liquid biopsy test for the diagnosis of bladder cancer. The liquid biopsy test is based on bladder cancer DNA methylation biomarkers developed... Read more
 
Copyright © 2000-2015 Globetech Media. All rights reserved.