We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics

BIOCHROM LTD

Biochrom Ltd. is a leading manufacturer of scientific instruments with over 40 years experience. The Biochrom Group c... read more Featured Products: More products

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

LAMP Technique Detects Toxoplasmosis

By LabMedica International staff writers
Posted on 16 Jun 2015
Print article
Image: The WPA-Biowave II Nano-spectrophotometer (Photo courtesy of Biochrom).
Image: The WPA-Biowave II Nano-spectrophotometer (Photo courtesy of Biochrom).
Image: Toxoplasma gondii tachyzoites from ascites fluid (Photo courtesy of Dr. L.L. Moore, Jr.).
Image: Toxoplasma gondii tachyzoites from ascites fluid (Photo courtesy of Dr. L.L. Moore, Jr.).
The analytical sensitivity and specificity of a newly described DNA amplification technique, loop-mediated isothermal amplification (LAMP), has been tested for the diagnosis of toxoplasmosis.

Toxoplasma gondii is an obligate intracellular protozoan parasite capable of infecting humans and all warm blooded animals worldwide and despite the high rate of infection in human, infections often remain asymptomatic in immunocompetent individuals.

Scientists at the Lorestan University of Medical Sciences (Khorramabad, Iran) prepared T. gondii tachyzoites from peritoneal exudate from infected mice. Extraction of DNA was performed using the classical phenol chloroform extraction, and two commercial DNA extraction kits. A Nano-spectrophotometer WPA-Biowave II (Biochrom; Cambridge, UK) was used to determine the concentration and quality of the DNA. Each T. gondii one 80-Mbp genome was assumed equal about 80 fg DNA.

The analytical sensitivity of LAMP and nested-polymerase chain reaction (PCR) was obtained against 10-fold serial dilutions of T. gondii DNA ranging from 1 ng to 0.01 fg. DNA samples of other parasites and human chromosomal DNA were used to determine the specificity of molecular assays. The team analyzed the sensitivity, specificity and technical performances of the LAMP and nested-PCR assays by targeting the two repetitive conserved regions (RE and B1) in the Toxoplasma genome for the molecular diagnosis of T. gondii infection.

After testing LAMP and nested-PCR in duplicate, the detection limit of RE-LAMP, B1-LAMP, RE-nested PCR and B1-nested PCR assays was one fg, 100 fg, 1 pg and 10 pg of T. gondii DNA respectively. All the LAMP assays and nested PCRs were 100% specific. The RE-LAMP assay was the most sensitive for the detection of T. gondii DNA.

The authors concluded the LAMP technique has a greater sensitivity for detection of T. gondii. Furthermore, these findings indicate that primers based on the RE are more suitable than those based on the B1 gene. However, the B1-LAMP assay has potential as a diagnostic tool for detection of T. gondii. Because of its simplicity, sensitivity, and specificity, LAMP is suggested as an appropriate method for the routine molecular diagnosis of toxoplasmosis. The study was published on May 20, 2015, in the Asian Pacific Journal of Tropical Medicine.

Related Links:

Lorestan University of Medical Sciences 
Biochrom 



Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
HLX
Complement 3 (C3) Test
GPP-100 C3 Kit
Gold Member
Xylazine Immunoassay Test
Xylazine ELISA

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: MOF materials efficiently enrich cfDNA and cfRNA in blood through simple operational process (Photo courtesy of Science China Press)

Blood Circulating Nucleic Acid Enrichment Technique Enables Non-Invasive Liver Cancer Diagnosis

The ability to diagnose diseases early can significantly enhance the effectiveness of clinical treatments and improve survival rates. One promising approach for non-invasive early diagnosis is the use... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.