Features | Partner Sites | Information | LinkXpress
Sign In
FOCUS DIAGNOSTICS, INC.
RANDOX LABORATORIES
GLOBETECH PUBLISHING

Whole-Genome Testing Helps Identify Breast Cancer Treatments

By Labmedica International staff writers
Posted on 26 Feb 2014
Image: The Qubit fluorometer (Photo courtesy of Life Technologies).
Image: The Qubit fluorometer (Photo courtesy of Life Technologies).
A molecular screening study has identified abnormalities in individual patients with breast cancer with the aim of providing targeted therapy matched to individuals' genomic alterations.

Progress in developing effective new chemotherapy or hormonal therapies for metastatic cancer has been slow, though there have been developments in therapies targeting specific genetic mutations in breast cancer.

In a multicenter, prospective trial scientists at the Institute Gustave Roussy (Villejuif, France) and colleagues from other institutions recruited 423 patients who had breast cancer with a metastasis accessible for biopsy in 18 centers in France, from June 16, 2011, to July 30, 2012. Comparative genomic hybridization (CGH) array and Sanger sequencing on phosphatidylinositol-4,5 -bisphosphate 3-kinase, (PIK3CA, exon 10 and 21) and v-akt murine thymoma viral oncogene homolog 1 (AKT1, exon 4) were used to assess metastatic biopsy samples from five medical centers.

DNA concentration was measured with Qubit 2.0 Fluorometer (Life Technologies; Paisley, UK) and sequencing was performed with Life Technologies' BigDye Terminator Cycle Sequencing Kit. For genomic analyses on microarray platforms (Agilent Technologies; Massy, France) tumor and control DNA were labeled by random priming, and then hybridized. To assess robustness of data, normal genomic DNA was used in all analyses to validate the genomic profile findings in tumor samples.

Examining the DNA from the samples, the team found that just under half (46%) of the patients in the study had a genomic alteration that could be targeted, while 39% had rare alterations for which no treatments currently exist. Therapy could be personalized in 55 (13%) of 423 patients. Of the 43 patients who were assessable and received targeted therapy, four (9%) had an objective response, and nine others (21%) had stable disease for more than 16 weeks.

Fabrice André MD, a professor and lead author said, "So far 55 of those enrolled, which is 28% of those with targetable alterations, have been matched with new treatments being tested in clinical trials. This emphasizes the need to increase the range of drug trials. Our goal is to have 30% of patients in clinical trials testing therapies targeting the alterations identified in their tumors." The study was published on February 7, 2014, in the journal Lancet Oncology.

Related Links:

Institute Gustave Roussy
Life Technologies
Agilent Technologies



EUROIMMUN AG
PURITAN MEDICAL
Sekisui Diagnostics
comments powered by Disqus
Life Technologies

Channels

Clinical Chemistry

view channel
Image: Enzyme-linked immunosorbent kit (ELISA) specific for human copeptin (Photo courtesy of USCN Life Science).

Preeclampsia Biomarker Detected Very Early In Pregnancy

A biomarker has been discovered that could give expecting mothers and their doctors the first simple blood test to reliably predict that a pregnant woman may develop preeclampsia, at least as early as... Read more

Pathology

view channel
Image: Glioblastoma multiforme (GBM) (Photo courtesy of the University of California, San Diego School of Medicine).

Brain Tumor Chemotherapy Biomarkers Identified

Cancer researchers have identified a new biomarker that they believe can predict whether glioblastoma multiformes (GBMs), the most common and aggressive type of malignant brain tumor, will be susceptible... Read more

Lab Tech.

view channel
Image: The ADVIA Centaur XPT Immunoassay System delivers the results that clinicians depend upon for accurate diagnoses and better patient care (Photo courtesy of Siemens Healthcare).

Introduction of New Central Laboratory Clinical Analyzers Highlights Recent Lab Equipment Exposition

An impressive array of new automated analyzers for central diagnostic laboratories was unveiled at a recent lab equipment trade fair. The IFCC WorldLab exposition held June 22-26, 2014, in Istanbul... Read more

Industry News

view channel

EKF Wins Distribution Contract and Regulatory Approval for Target Regions

EKF Diagnostics Holdings (Cardiff, Wales, UK), an AIM-listed point-of-care, central laboratory and molecular diagnostics business, announced that it has signed a new distribution contract in China and that one of its OEM (original equipment manufacturer) partners has received further regulatory approval across several target... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.