Features | Partner Sites | Information | LinkXpress
Sign In
GLOBETECH PUBLISHING LLC
RANDOX LABORATORIES
FOCUS DIAGNOSTICS, INC.

Whole-Genome Testing Helps Identify Breast Cancer Treatments

By Labmedica International staff writers
Posted on 26 Feb 2014
Image: The Qubit fluorometer (Photo courtesy of Life Technologies).
Image: The Qubit fluorometer (Photo courtesy of Life Technologies).
A molecular screening study has identified abnormalities in individual patients with breast cancer with the aim of providing targeted therapy matched to individuals' genomic alterations.

Progress in developing effective new chemotherapy or hormonal therapies for metastatic cancer has been slow, though there have been developments in therapies targeting specific genetic mutations in breast cancer.

In a multicenter, prospective trial scientists at the Institute Gustave Roussy (Villejuif, France) and colleagues from other institutions recruited 423 patients who had breast cancer with a metastasis accessible for biopsy in 18 centers in France, from June 16, 2011, to July 30, 2012. Comparative genomic hybridization (CGH) array and Sanger sequencing on phosphatidylinositol-4,5 -bisphosphate 3-kinase, (PIK3CA, exon 10 and 21) and v-akt murine thymoma viral oncogene homolog 1 (AKT1, exon 4) were used to assess metastatic biopsy samples from five medical centers.

DNA concentration was measured with Qubit 2.0 Fluorometer (Life Technologies; Paisley, UK) and sequencing was performed with Life Technologies' BigDye Terminator Cycle Sequencing Kit. For genomic analyses on microarray platforms (Agilent Technologies; Massy, France) tumor and control DNA were labeled by random priming, and then hybridized. To assess robustness of data, normal genomic DNA was used in all analyses to validate the genomic profile findings in tumor samples.

Examining the DNA from the samples, the team found that just under half (46%) of the patients in the study had a genomic alteration that could be targeted, while 39% had rare alterations for which no treatments currently exist. Therapy could be personalized in 55 (13%) of 423 patients. Of the 43 patients who were assessable and received targeted therapy, four (9%) had an objective response, and nine others (21%) had stable disease for more than 16 weeks.

Fabrice André MD, a professor and lead author said, "So far 55 of those enrolled, which is 28% of those with targetable alterations, have been matched with new treatments being tested in clinical trials. This emphasizes the need to increase the range of drug trials. Our goal is to have 30% of patients in clinical trials testing therapies targeting the alterations identified in their tumors." The study was published on February 7, 2014, in the journal Lancet Oncology.

Related Links:

Institute Gustave Roussy
Life Technologies
Agilent Technologies



CELLAVISION AB
MEDLAB Asia
KARL HECHT GMBH & CO KG
PERIPHERAL VISIONS INC

Channels

Hematology

view channel

PROPPR Trial Supports Use of Transfusion Protocol Initially Developed for Battlefield Trauma Patients

In a groundbreaking multisite prospective clinical trial, the damage control resuscitation (DCR) protocol for massive blood transfusion significantly improved severe trauma patient outcome from hemorrhagic shock, when compared to another commonly used protocol. DCR, used in most hospitals today, was first developed by... Read more

Lab Tech.

view channel
Image:  The Flame – a miniature spectrometer for medical diagnostics, biotechnology, and life sciences, that brings flexible optical sensing power to laboratory and point-of-care instrument integration (Photo courtesy of Ocean Optics).

Next Generation Miniature Spectrometer Brings Flexibility to Diagnostics and Biotechnology

The mini spectrometer combines decades of pioneering miniature spectrometer design expertise with leading techniques, adding flexible optical sensing power to laboratory and point-of-care instrument integration.... Read more

Industry News

view channel

Healthcare Revolution Predicted with Rise of Genomic Testing for Personalized Medicine

A new Frost & Sullivan (Mountain View, CA, USA) analysis has found that the direct-to-individual sector of genomic testing is now thriving, though not without criticism about the potential medical interpretation of some forms of tests that may have been initially requested for nonmedical purposes, such as those for... Read more
 

Events

08 Mar 2015 - 12 Mar 2015
18 Mar 2015 - 20 Mar 2015
18 Mar 2015 - 18 Mar 2015
Copyright © 2000-2015 Globetech Media. All rights reserved.