Features | Partner Sites | Information | LinkXpress
Sign In
FOCUS DIAGNOSTICS, INC.
SEEGENE
RANDOX LABORATORIES

Biomarkers Help Predict Outcome in Deadly Lung Disease

By Labmedica International staff writers
Posted on 15 Oct 2013
Image: Histopathology of idiopathic pulmonary fibrosis (Photo courtesy of the American Thoracic Society).
Image: Histopathology of idiopathic pulmonary fibrosis (Photo courtesy of the American Thoracic Society).
A gene expression profile has been identified that can predict outcomes and lead to better treatment for one of the most lethal lung diseases, idiopathic pulmonary fibrosis (IPF).

In most cases of IPF the cause cannot be identified, and there is no cure other than a lung transplant and while some patients experience a progressive course that leads to death within one to two years, others experience a relative stable disease.

Scientists from Yale University (New Haven, CT, USA) with colleagues from other institutions recruited a discovery cohort of 45 patients from the University of Chicago (IL, USA) and 75 patients in a replication cohort from the University of Pittsburgh (PA, USA). The goal of the study was to identify changes in expression of genes in the blood that are predictive of poor outcomes among patients with IPF.

The teams analyzed the expression of the genes in the whole genome of patients with IPF, using peripheral blood mononuclear cell (PBMC). They identified 52 genes that significantly correlated with outcome. They further found that the decreased expression of four genes that encode for cluster of differentiation 28 (CD28), inducible T-cell co-stimulator (ICOS), lymphocyte-specific protein tyrosine kinase (LCK), and interleukin 2 (IL2)-inducible T-cell kinase (ITK). These genes predicted shorter survival time in patients with IPF.

The investigators believe the discovery of these biomarkers will help physicians better predict disease presence, severity, and prognosis in IPF patients. Naftali Kaminski, MD, a professor at Yale School of Medicine and a senior author of the study said, “Given the fact that lung transplantation is the only therapy that has shown to improve survival in IPF, our test could allow physicians to refer IPF patients for lung transplant at the right time, not too late and not too early.”

The author concluded that microarray-derived 52-gene expression profile or quantitative reverse transcription polymerase chain reaction (qRT-PCR) of CD28, ICOS, LCK, and ITK members of this signature was sufficient to identify IPF patients destined for poor outcomes. Combining gene expression data with clinical parameters enhanced outcome prediction; thus, the results could have considerable value in clinical evaluations and management of patients with this devastating lung disease. The study was published on October 2, 2013, in the journal Science Translational Medicine.

Related Links:
Yale University
University of Chicago
University of Pittsburgh



ADVANCED INSTRUMENTS
GREINER-BIO-ONE
PURITAN MEDICAL
BioConferenceLive

Channels

Clinical Chemistry

view channel
Image: The ICON 25 hCG point-of-care pregnancy test (Photo courtesy of Beckman Coulter).

Pregnancy Tests Evaluated During Early Gravidity

The predominant hormone human chorionic gonadotropin (hCG) variant in urine, hCG β core fragment (hCGβcf), has been demonstrated to cause false-negative results in qualitative point-of-care (POC) hCG devices.... Read more

Hematology

view channel

Blood Donations Screened for Viruses Using Multiplex Assay

A global healthcare company was chosen to partner the Japanese Red Cross (JRC; Tokyo, Japan) for nucleic acid screening of the nation's blood supply. Under the terms of the 7-year agreement, the healthcare company, Grifols (Barcelona, Spain) will provide the JRC with its newest automation platform, the Procleix Panther... Read more

Pathology

view channel
Image: Immunostaining of human lung tissue, fixed with HOPE, from a patients suffering from legionnaire\'s disease. One legionella protein (red-brown), the bacteria-containing vacuoles and individual legionella inside the scavenger cells can be detected. The infection process can be observed immediately using proteomics (Photo courtesy of Braunschweig University of Technology).

Novel Method Prepares Tissue Samples for Analyzing Proteins

A new way of preparing patient tissue for analyses might soon become the new standard, possibly replacing tissues fixed with formalin, before they are embedded in wax-like paraffin and cut into razor-thin slices.... Read more

Industry News

view channel

Roche and Hitachi Renew Alliance in Diagnostics

Roche (Basel, Switzerland) and Hitachi (Tokyo, Japan) have renewed their alliance in the diagnostics business by 10-years for the joint development and manufacture of the next generation of instruments and workflow automation solutions for medical laboratories. The renewed alliance marks a significant milestone towards... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.