Features | Partner Sites | Information | LinkXpress
Sign In
FOCUS DIAGNOSTICS, INC.
GLOBETECH PUBLISHING LLC
RANDOX LABORATORIES

Biomarkers Identified for Acute Kidney Injury Risk

By Labmedica International staff writers
Posted on 28 Feb 2013
Two biomarkers have been discovered for the early assessment of acute kidney injury (AKI) that can be easily measured in urine.

The two novel biomarkers can detect affected patients roughly 12 to 36 hours earlier than current tests for adult patients and have been subsequently validated using a clinical assay and compared to existing markers of AKI.

Scientists at the Mayo Clinic (Rochester, MN, USA) led a multicenter study to evaluate nearly 340 biomarkers to find the two with the highest correlation to kidney injury risk. In the discovery phase, they enrolled 522 adults in three distinct cohorts including patients with sepsis, shock, major surgery, and trauma and examined over 300 markers. In the validation study, they enrolled 744 adult subjects with critical illness and without evidence of AKI at enrollment. The final analysis cohort was a heterogeneous sample of 728 critically ill patients.

Paired urine and blood samples were collected at enrollment and up to 18 hours later by standard methods and centrifuged. Biomarkers were measured with single or multiplexed immunoassays using standard enzyme-linked immunosorbent assays (ELISA). The platforms used were the Luminex 200 (Luminex; Austin, TX, USA); the MSD SECTOR Imager 6000 (Meso Scale Discovery; Gaithersburg, MD, USA), or the Astute140 Meter (Astute Medical; San Diego, CA, USA). The two biomarkers with the highest correlation to kidney injury risk were the Insulin Growth Factor Binding Protein-7 (IGFBP-7) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2).

The investigators also examined the performance of urine TIMP-2 and IGFBP7 compared to various other markers including urine kidney-injury marker-1 (KIM-1) and urine neutrophil gelatinase-associated lipocalin (NGAL) in terms of discrimination between AKI of different severities and various non-AKI conditions including chronic kidney disease. Unlike existing markers, TIMP-2 and IGFBP7 showed clear separation between AKI and non-AKI conditions.

The authors concluded that urine insulin-like growth factor-binding protein 7 (IGFBP7) and tissue inhibitor of metalloproteinases-2 (TIMP-2) are new biomarkers for AKI and perform better than existing markers for predicting the development of moderate or severe AKI within12 hours of sample collection. The risk for major adverse kidney events, such as death, dialysis, or persistent renal dysfunction within 30 days, was elevated sharply for [TIMP-2]•[IGFBP7] above 0.3 and doubled when values were greater than 2.0. The study was published on February 6, 2013 in the journal Critical Care.

Related Links:
Mayo Clinic
Luminex
Meso Scale Discovery



KARL HECHT GMBH & CO KG
CELLAVISION AB
MEDLAB Asia

Channels

Genetic Tests

view channel
Image: For each chromosome, key SNPs are displayed above the chromosome and non-key SNPs below the chromosome, enabling users to determine the accuracy of haploblock calling. SNPs failed to be called are displayed as white points in the middle of the chromosome (Photo courtesy of Illumina).

Karyomapping Proposed as Alternative to Preimplantation Genetic Diagnosis for Evaluation of IVF Embryos

British researchers have suggested that karyomapping is a viable and cost-effective method for detecting a wide range of genetic diseases in IVF (in vitro fertilization) embryos. Preimplantation Genetic... Read more

Industry News

view channel
Image: Positive case of lung tissue stained for ALK with VENTANA ALK (D5F3) CDx Assay, a companion diagnostic immunohistochemical test in the final stages of the FDA premarket approval process (Photo courtesy of Ventana Medical Systems and PRNewsFoto).

Premarket Approval Sought for CDx Test to Benefit Lung Cancer Patients

Ventana Medical Systems, Inc. (Tucson, AZ, USA) has announced its US Food & Drug Administration (FDA) final stage submission for premarket approval (PMA) of its companion diagnostic (CDx) immunohistochemistry... Read more
 

Events

05 Mar 2015 - 08 Mar 2015
18 Mar 2015 - 20 Mar 2015
Copyright © 2000-2015 Globetech Media. All rights reserved.