Features | Partner Sites | Information | LinkXpress
Sign In
FOCUS DIAGNOSTICS, INC.
AB Sciex
RANDOX LABORATORIES

Biomarkers Identified for Acute Kidney Injury Risk

By Labmedica International staff writers
Posted on 28 Feb 2013
Two biomarkers have been discovered for the early assessment of acute kidney injury (AKI) that can be easily measured in urine.

The two novel biomarkers can detect affected patients roughly 12 to 36 hours earlier than current tests for adult patients and have been subsequently validated using a clinical assay and compared to existing markers of AKI.

Scientists at the Mayo Clinic (Rochester, MN, USA) led a multicenter study to evaluate nearly 340 biomarkers to find the two with the highest correlation to kidney injury risk. In the discovery phase, they enrolled 522 adults in three distinct cohorts including patients with sepsis, shock, major surgery, and trauma and examined over 300 markers. In the validation study, they enrolled 744 adult subjects with critical illness and without evidence of AKI at enrollment. The final analysis cohort was a heterogeneous sample of 728 critically ill patients.

Paired urine and blood samples were collected at enrollment and up to 18 hours later by standard methods and centrifuged. Biomarkers were measured with single or multiplexed immunoassays using standard enzyme-linked immunosorbent assays (ELISA). The platforms used were the Luminex 200 (Luminex; Austin, TX, USA); the MSD SECTOR Imager 6000 (Meso Scale Discovery; Gaithersburg, MD, USA), or the Astute140 Meter (Astute Medical; San Diego, CA, USA). The two biomarkers with the highest correlation to kidney injury risk were the Insulin Growth Factor Binding Protein-7 (IGFBP-7) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2).

The investigators also examined the performance of urine TIMP-2 and IGFBP7 compared to various other markers including urine kidney-injury marker-1 (KIM-1) and urine neutrophil gelatinase-associated lipocalin (NGAL) in terms of discrimination between AKI of different severities and various non-AKI conditions including chronic kidney disease. Unlike existing markers, TIMP-2 and IGFBP7 showed clear separation between AKI and non-AKI conditions.

The authors concluded that urine insulin-like growth factor-binding protein 7 (IGFBP7) and tissue inhibitor of metalloproteinases-2 (TIMP-2) are new biomarkers for AKI and perform better than existing markers for predicting the development of moderate or severe AKI within12 hours of sample collection. The risk for major adverse kidney events, such as death, dialysis, or persistent renal dysfunction within 30 days, was elevated sharply for [TIMP-2]•[IGFBP7] above 0.3 and doubled when values were greater than 2.0. The study was published on February 6, 2013 in the journal Critical Care.

Related Links:
Mayo Clinic
Luminex
Meso Scale Discovery



PURITAN MEDICAL
77 ELEKTRONIKA
DiagCor Bioscience
comments powered by Disqus
Sekisui Diagnostics

Channels

Genetic Tests

view channel
Image: The BioMark HD and EP1 platform with Digital Array Integrated Fluidic Circuits for quantitative real-time polymerase chain reaction (Photo courtesy of Fluidigm).

Molecular Test Identifies Acute Transplant Rejection

The development of noninvasive molecular assays to improve disease diagnosis and patient monitoring is a critical need in renal transplantation, as acute rejection (AR) increases the risk for chronic graft... Read more

Microbiology

view channel
Image: The causative parasite of Chagas disease Trypanosoma cruzi in a blood film (Photo courtesy of the CDC - US Centers for Disease Control and Prevention).

Undiagnosed Chagas Disease Emerging as Public Health Threat

Chagas disease or American trypanosomiasis is typically spread to people through the feces of blood-sucking triatomine bugs sometimes called kissing bugs because they feed on people's faces during the night.... Read more

Pathology

view channel

Identification of Independent Primary Lung Tumors and Tumors with Metastases Using Next-Generation DNA Sequencing

A team of oncologists and surgeons used a next-generation DNA sequencing (NGS) technique to determine whether non-small-cell lung cancer patients with multiple tumors carried independent primary tumors or a single primary tumor that had metastasized. For patients with multiple tumors, distinguishing independent primary... Read more

Industry News

view channel

Oncology Companion Diagnostics Sector Grows to Over USD 1 Billion

The market for diagnostic test and pharmaceutical drug pairs for cancer treatment has grown significantly, according to the report “Companion Diagnostic Markets” from healthcare market research firm Kalorama Information (New York City, NY, USA). The global companion diagnostics (CDx) market is valued at about USD 1.... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.