Features | Partner Sites | Information | LinkXpress
Sign In
RANDOX LABORATORIES
Bio-Rad Diabetes Division
FOCUS DIAGNOSTICS, INC.

Lung Biopsies Reduced by Panel of Serum Biomarkers

By Labmedica International staff writers
Posted on 04 Apr 2012
A panel of serum biomarkers could help predict the level of lung cancer risk in high-risk patients, offering doctors an option before proceeding with a biopsy.

The panel of protein biomarkers could help in lung cancer diagnosis as given the substantial risks of invasive diagnostic thoracic procedures, and unselective biopsy of every person with a small nodule is clinically undesirable.

A multicenter team, led by scientists at the University of Pittsburgh Cancer Institute (PA, USA), identified candidate serum biomarkers in a training set of sera from 56 patients with biopsy-proven primary non-small-cell lung cancer and 56 age-, sex-, and smoking-matched controls. They measured 70 cancer-related proteins by Luminex xMAP multiplexed immunoassays. The classification performance of the 10-biomarker panel was also analytically validated using enzyme-linked immunosorbent assays (ELISA) in a second independent case/control population, further validating the robustness of the panel.

The scientists identified a panel of 10 serum biomarkers including prolactin, transthyretin, thrombospondin-1, E-selectin, C-C motif chemokine 5, macrophage migration inhibitory factor, plasminogen activator inhibitor, receptor tyrosine-protein kinase, cytokeratin fragment 21.1, and serum amyloid A, which distinguished lung cancer patients from controls. The xMAP-multiplexed immunoassays are products of Luminex (Austin, TX, USA).

The ELISA kits for human thrombospondin-1, plasminogen activator inhibitor (PAI-1 and SERPINE1), and macrophage migration inhibitory factor (MIF), are products of R&D Systems (Minneapolis, MN, USA). ELISA kits for carcinoembryonic antigen (CEA), receptor tyrosine-protein kinase 2, and transthyretin (TTR) are manufactured by Immunology Consultants Laboratory, (Minneapolis, MN, USA), Calbiochem (Gibbstown, NJ, USA), and ALPCO (Salem, NH, USA), respectively.

The authors concluded that although the biomarker model they described could not detect every lung cancer, it offers a significant clinical improvement over computed tomography (CT) imaging alone. According to the study, about 20% of the 1-2 cm nodules that are concerning enough to be considered for biopsy are actually malignant, but those who are identified by small pulmonary nodules can be correctly classified by the study's model. The article was published in the April 2012 issue of the Journal of Thoracic Oncology.

Related Links:

University of Pittsburgh Cancer Institute
Luminex
R&D Systems




PURITAN MEDICAL
77 ELEKTRONIKA
DiagCor Bioscience
comments powered by Disqus
Sekisui Diagnostics

Channels

Genetic Tests

view channel
Histopathology of colonic adenocarcinoma; the glands are enlarged and filled with necrotic debris (Photo courtesy of the University of Utah).

Promising Blood Biomarkers Isolated for Colorectal Cancer

The search for blood-borne biomarkers that could be used to screen for colorectal cancer (CRC) has uncovered two promising candidates that may one day lead to the development of a simple blood test.... Read more

Microbiology

view channel

Novel Sequence Analysis Services Improve Pathogen Identification

A set of new-generation sequencing (NGS) services provides unique tools for more effective identification of known and unknown microorganisms, including bacterial, viral, fungal, and protozoan parasite pathogens. Aperiomics (Ashburn, VA, USA) has now introduced its breakthrough Absolute-NGS Pathogen Detection Platform... Read more

Industry News

view channel

Market for qPCR Exceeds USD 3 Billion

The market for real-time quantitative polymerase chain reaction (qPCR) was valued at about USD 3.2 billion for 2013 according to Kalorama Information (New York, NY, USA) an increase from USD 2.8 billion in 2011. The healthcare market researcher said that the testing system is considered the workhorse of research providing... Read more
 
Copyright © 2000-2014 Globetech Media. All rights reserved.