We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
INTEGRA BIOSCIENCES AG

Sciex

SCIEX develops and sells scientific instrumentation, software, and services for the life science, clinical research, ... read more Featured Products: More products

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Novel Candidate Protein Biomarkers Identified for Gastric Cancer

By LabMedica International staff writers
Posted on 25 Mar 2014
Print article
Image: Ultra nano-liquid chromatography system (Photo courtesy of Eksigent).
Image: Ultra nano-liquid chromatography system (Photo courtesy of Eksigent).
The early detection of stomach or gastric cancer (GC) has been revealed with the identification of four new biomarkers in the blood of human cancer patients.

This poor outcome of GC can be attributed to an extended asymptomatic period associated with this cancer, and difficulty in the detection of early stage gastric adenocarcinoma when treatment could improve long term survival of patients.

Scientists at the University of Adelaide (Australia) collected serum samples from 37 preoperative GC patients with intestinal type gastric adenocarcinoma. There were 10 females, with a mean age range of 69 ± 10 years and 17 males with a mean age range of 66 ± 11 years. In the cohort were 11 early stage cancer patients, and the controls included healthy and noncancerous patients with other gastric disease.

Enzyme-linked immunosorbent assays (ELISA) were performed for four proteins afamin, clusterin, haptoglobin and vitamin D binding protein (VDBP) and obtained from USCN Life Science Inc., (Wuhan, China). Ten GC and 10 controls serum samples were analyzed with multiple reaction monitoring high resolution mass spectrometry (MRM-HR). These serum samples were run on Triple TOF 5600 mass spectrometer (AB Sciex; Framingham, MA, USA) with an Ultra nano-liquid chromatography (LC) system (Eksigent; Redwood City, CA, USA).

The analysis of the ELISA results showed that the sensitivity and specificity values represented as Area Under the Curve (AUC) were: 0.94 for clusterin, 0.84 for VDBP, 0.58 for haptoglobin, and 0.52 for afamin. The MRM-HR data when analyzed revealed a trend for differential regulation of afamin, clusterin and VDBP. MRM-HR analysis revealed a significant higher abundance of haptoglobin in GC patients which was similar to that observed in the results from the ELISA. All four proteins were individually superior to a current clinical marker CA72-4 in discriminating stomach cancer from healthy controls.

The authors concluded that the differential regulation of four serum proteins in gastric cancer patients compared with normal healthy individuals were found with clusterin, VDBP and afamin down-regulated, and haptoglobin up-regulated in serum from GC versus benign GI disease cases. Peter Hoffmann, PhD, a professor at the University of Adelaide and senior author of the study said, “Stomach cancer is typically without symptoms in the early stages so most cancers are not diagnosed until the later stages, and the survival rates are therefore low. A noninvasive, inexpensive screening technique through a simple blood test for the early detection of stomach cancer would make a huge difference in the survival outcomes for people with this disease.” The study was published on February 7, 2014, in the journal Biochimica et Biophysica Acta.

Related Links:

University of Adelaide
AB Sciex
Eksigent


Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
One Step HbA1c Measuring System
GREENCARE A1c
POCT Fluorescent Immunoassay Analyzer
FIA Go
Gold Member
Real-time PCR System
GentierX3 Series

Print article

Channels

Molecular Diagnostics

view channel
Image: The cobas Malaria test is the first FDA-approved molecular test to screen U.S. blood donors for malaria (Photo courtesy of Roche)

First FDA-Approved Molecular Test to Screen Blood Donors for Malaria Could Improve Patient Safety

Malaria, a serious illness that often leads to death, is spread by a specific mosquito species that infect humans with a parasite. Other transmission modes include blood transfusions, organ transplants,... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The medical office procedure detects the key biomarker in Parkinson’s and related neurodegenerative diseases (Photo courtesy of BIDMC)

Simple Skin Biopsy Test Detects Parkinson’s and Related Neurodegenerative Diseases

Parkinson's disease and a group of related neurodegenerative disorders known as synucleinopathies impact millions globally. These conditions, including Parkinson’s disease (PD), dementia with Lewy bodies... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.